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鹅细小病毒VP1基因的克隆及序列分析
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马波， 王君伟， 李洪涛， 刘宝全
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（东北农业大学 动物医学院， 黑龙江 哈尔滨150030）
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摘要：
参照GenBank中收录的鹅细小病毒(GPV)B株基因序列设计并合成了扩增GPV H1株VP1的1对引物，利用PCR技术扩增出长约2.2kb的目的片段，将其克隆到pMD18T载体上，进行了序列测定及分析。测序结果表明，GPV H1株VP1基因由2199个核苷酸组成，编码732个氨基酸。经与B株、YG株进行同源性比较，核苷酸的同源性分别为98.50%和93.18%；推导的氨基酸同源性分别为98.09%和95.77%。 关键词：
鹅细小病毒； VP1基因； 克隆； 序列分析
中图分类号：S 852.659.1：Q 819 文献标识码：
A 文章编号：1000-6419(2003)12-0007-04 |
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Cloning and sequence analysis of VP1 gene of goose parvovirus
MA Bo， WANG Junwei， LI Hongtao， LIU Baoquan
(College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030，China) |
Abstract: A pair of primers was designed to amplify VP1 gene of GPV by PCR according to the published sequence of GPV B strain in GenBank. The product of PCR whose length is 2.2 kb was cloned to the
pMD18T vector，then made sequencing and analyzing . The result of sequence showed that VP1 gene included
2199 bp, which encoded 732 amino acids. The gene shared 98.50% and 93.18% homology in bases, and shared 98.09% and 95.77% respectively in amino acid with GPV strains B and YG.
Key words: GPV； VP1 gene； cloning； sequence analysis |
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