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鸡传染性支气管炎病毒SC株S1基因的序列分析

刘洪义1， 刘兴友2

（1. 西南科技大学， 四川 绵阳621000； 2. 郑州牧业工程高等专科学校， 河南 郑州450011）

摘要： 用RTPCR扩增了鸡传染性支气管炎病毒(IBV)SC株S1基因，连接到pMD18T载体上，克隆后进行了核酸序列分析，证实SC株S1基因与基因库中收录的国外毒株H120和M41的同源性较低，分别为81.1%和80.0%，而与国内JX9901株的同源性达到91.3%，初步证实国内存在IBV新毒株。

关键词： 鸡传染性支气管炎病毒； S1基因； 核酸序列

中图分类号：S 852.659.6  文献标识码：A  文章编号：1000-6419(2003)12-0047-04

Sequence analysis of S1 gene of infectious bronchitis virus SC strain

LIU Hongyi1， LIU Xingyou2

(1. Southwest Science and Technology University, Mianyang 621000，China; 
2. Zhengzhou College of Animal Husbandry and Engineering, Zhengzhou 450011, China)

Abstract: S1 gene of infectious bronchitis virus (IBV) SC strain was amplified by RTPCR and sequenced by automatic sequencing system. The sequence consisted of 1737bp. When S1 gene of SC strain was compared with that of H120, M41 or JX9901 strain, which have been published previously in GenBank, homologies between them were 81.1%, 80.0% or 91.3%, respectively. The results showed that the SC strain is a new IBV strain in China.

Key words: infectious bronchitis virus； S1 gene； DNA sequence 

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