欢迎访问《中国兽医科技》网站 -- www.cjvst.com　　　　　　　　　　

　 　 　


应用PCR检测山羊痘病毒

郭巍1， 陈杰2， 黄保续2， 李晓成2， 吴发兴2， 张燕霞2， 李一经1

（1. 东北农业大学 动物医学院， 黑龙江 哈尔滨150030； 2. 农业部动物检疫所 国家动物流行病学研究中心， 山东 青岛266032）

摘要： 初步建立了用于诊断山羊皮肤组织中山羊痘病毒的PCR方法。试验设计了2对引物，用以分别检测山羊痘病毒的特异性基因和α微管蛋白基因。克隆到的DNA扩增产物的序列与GenBank中收录的序列同源性达到98%。试验表明，用PCR方法可快速检测样品中的山羊痘病毒。

关键词： 山羊痘病毒； PCR； P32基因

中图分类号：S 858.26 文献标识码： B 文章编号：1000-6419(2003)12-0050-03

Detection of capripoxvirus in goat skin specimen by PCR

GUO Wei1, CHEN Jie2, HUANG Baoxu2, LI Xiaocheng2, 
WU Faxing2, ZHANG Yanxia2, LI Yijing1

(1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150001,China; 
2. National Research Center of Epizootiology, Animal Quarantine Institute of Ministry of Agriculture, Qingdao 266032,China)

Abstract: A method of polymerase chain reaction was established in this experiment in order to rapidly detect and diagnose capripoxvirus in goat skin samples. Two primer pairs were designed, one primer pair for amplification of the special gene P32 of capripoxvirus and the other for the gene of αtublin in the skin. The PCR product was sequenced and compared with the published sequence of P32 gene in GenBank. Other specimen were examined by the PCR and the same results with clinical diagnosis were got. The result indicated that the PCR can detect capripoxvirus in goat in a short time.

Key words: capripoxvirus; PCR; P32 gene

《中国兽医科技》 © 版权所有
Chinese Journal of Veterinary Science and Technology(CJVST)
webmaster@cjvst.com