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少孢节丛孢菌孢子批量培养及通过动物消化道的试验

杨莲茹， 考桂兰， 杨晓野， 刘珍莲， 李晨

(内蒙古农业大学 动物科学与医学学院， 内蒙古 呼和浩特010018)

摘要： 试验了捕食线虫性真菌——少孢节丛孢菌（Arthrobotrys oligospora）孢子批量培养技术、孢子通过兔消化道后的活力及其对绵羊消化液的体外耐受力。结果表明，少孢节丛孢菌孢子最适合以玉米粒为基质批量培养；少孢节丛孢菌的孢子在通过兔消化道后，可以保持存活；并且在体外对绵羊瘤胃液、真胃液、十二指肠液有一定的耐受力。

关键词： 捕食线虫性真菌； 少孢节丛孢菌； 线虫； 分生孢子

中图分类号：S 852.66  文献标识码： B  文章编号：1000-6419(2004)01-0050-04

Cultivation of batches of Arthrobotrys oligospora conidia and experiment of their passing through alimentary canal in animals

YANG Lianru, KAO Guilan, YANG Xiaoye, LIU Zhenlian, LI Chen

(College of Animal Science and Medicine, Nei Monggol Agricultural University, Hohhot 010018, China)

Abstract: he technique of cultivating conidia of Arthrobotrys oligospora in batches, their trapping activity after going through the alimentary canal of rabbits and their endurance in the digestive fluid of sheep in vitro were carried out. The results indicated that the best medium was maize grains for cultivating conidia of Arthrobotrys oligospora in batches. It was also showed that the conidia can be able to survive after passing through the gastrointestinal tract of rabbits and endure in the fluid of rumem, abomasums and duodenum of sheep in vitro.

Key words: nematodetrapping fungi; Arthrobotrys oligospora; nematodes; conidia

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