|
Immune response of DNA vaccine against footandmouth
disease virus in cavians enhanced by 3D protein expressed in the Pichia pastoris secreted expression system
GUO Huichen1, SUN Shiqi1, YUN Tao2 , ZHANG
Tengguo3,
BAO Huifang1, CHEN Yingli1, MA Junwu1, LIU
Xiangtao1,
LIU Zaixin1, XIE Qingge1
(1. Key Laboratory of Animal Virology, Ministry of Agriculture/Lanzhou Veterinary Research
Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China; 2. College of
Animal Science and Technology , Shihezi University , Shihezi 832003, China; 3. College of
Life Science, Lanzhou University, Lanzhou 730046, China） |
Abstract: In order to evaluate the enhanced immune response of the footandmouth disease virus (FMDV) DNA vaccine, which was designed to produce viral capsids lacking infectious viral nucleic acid, the gene P12X3C including full length P1, 2A, 3C and a part of 2B was constructed. FMDV 3D protein, which was expressed in the Pichia pastoris secreted expression system and was purified, was injected with
pcDNA3.1/P12X3C. The antiFMDV antibodies were detected by indirect ELISA. The T lymphocyte proliferation response was tested by MTT assay. The neutralization antibodies titers were analyzed by microneutralization assay .The results indicated that antiFMDV antibodies were elicited and increased by plasmid DNA in the second week after vaccination, that the neutralization antibodies were induced with high level and that the T lymphocyte proliferation response was enhanced after vaccination. In the challenge test, all cavians immunized with pcDNA3.1/P12X3Cwere fully protected againstFMDV challenge, but the result obtained from the group injected with 3D protein together plasmid pcDNA3.1/P12X3C wasnot satisfying. In conclusion, the results encouraged further work towards the development of DNA vaccines against FMDVand providedthe basis of research for DNA vaccines.
Key words: footandmouth disease virus (FMDV); DNA vaccine; gene vaccination |
