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柔嫩艾美球虫抗药株第二代裂殖子的双向电泳图谱比较

刘文江1,2， 黄兵2， 欧阳五庆1， 韩红玉2， 程国锋2， 陈兆国2， 赵其平2

（1. 西北农林科技大学 动物科技学院， 陕西 杨凌712100； 2. 中国农业科学院 上海家畜寄生虫病研究所 农业部动物寄生虫学重点开放实验室， 上海200232）

摘要： 对实验室诱导的柔嫩艾美球虫马杜拉霉素、地克珠利抗药株及其同母源敏感株第二代裂殖子的蛋白质进行双向电泳，构建了稳定的双向电泳图谱。经Imagemaster 2D Elite软件分析，敏感株和抗药株平均可分离900个点；以敏感株平均胶作为参考胶，抗药株平均胶与之比较，得到差异蛋白质斑点，其中地克珠利抗药株和马杜拉霉素抗药株各有4个表达差异点。这些差异表达的蛋白质可能参与了球虫抗药性产生的过程。

关键词： 柔嫩艾美球虫； 蛋白质组； 双向电泳； 抗药性； 第二代裂殖子

中图分类号：S 852.723  文献标识码： A  文章编号：1000-6419(2004)03-0021-05

Analysis of different expressed proteins of second generationmerozoites of Eimeria tenella drugresistance strainsby twodimensional electrophoresis

LIU Wenjiang1,2， HUANG Bing2, OUYANG Wuqing1, HAN Hongyu2,
CHENG Guofeng2, CHEN Zhaoguo2, ZHAO Qiping2

(1. College of Animal Science and Technology, Northwest SciTech University of Agriculture and
Foresty, Yangling 712100,China; 2. Key Laboratory of Animal Parasitology, Ministry of 
Agriculture/Shanghai Institute of Domestic Animal Parasitology, Chinese Academy 
of Agricultural Sciences,Shanghai 200232,China)

Abstract: The proteins of Eimeria tenella sensitive strain, diclazurilresistance strain and maduramasinresistance strain induced in our laboratory were extracted by Tris(pH9.5)and were analysed by the twodimensional electrophoresis. The 2DE gel had been prepared by displaying the protein component of the second generation merozoites of Eimeria tenella. The silve stained images were compared after analysis using the 2DE Imagemaster Elite 3.01.About 900 spots were obtained from the sensitive strain and the drugresistance strains. Four different expressed spots were shown from the diclazuriresistance strain and the maduramasinresistance strain respectively. These different expressed protein spots maybe were involved in the development of drug resistance.

Key words: Eimeria tenella; proteome; twodimensional electrophoresis; drug resistance; second generation merozoite

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