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应用PCRRFLP分析鉴别广西鸡毒霉形体

邓显文， 谢芝勋， 谢志勤， 唐小飞， 刘加波， 廖敏， 庞耀珊

（广西兽医研究所， 广西 南宁 530001）

摘要： 针对鸡毒霉形体（MG）16S和23S rRNA基因间的高变区序列设计合成了1对引物，对MG菌株进行了PCR扩增，获得了1条899bp的DNA片段，而其他禽病病原DNA无扩增。扩增产物经用限制性内切酶AfaⅠ和DraⅠ进行酶切片段长度多态性分析，表明各MG菌株酶切图谱间均存在差异。

关键词： 鸡毒霉形体； PCR； 限制性片段长度多态性； 广西

中图分类号：S 852.62  文献标识码： B  文章编号：1000-6419(2004)03-0041-04

Analysis and identification of Mycoplasma gallisepticum 
from Guangxi Region by PCRRFLP

DENG Xianwen, XIE Zhixun, XIE Zhiqin， TANG Xiaofei,
LIU Jiabo, LIAO Min, PANG Yaoshan

(Guangxi Veterinary Research Institute, Nanning 530001,China)

Abstract: According to Mycoplasma gallisepticum (MG) hypervariable region between 16S rRNA and 23S rRNA, a pair of primers was designed. The MG DNA fragment 899bp in length was specifically amplified by means of using the primers. The PCR product was analysed by the restriction fragment length polymorphism (RFLP) method. The results indicated that the RFLP patterns of the amplified fragment were different. The current study may play important role in MG epidemiological investigation in Guangxi Region.

Key words: PCR； restriction fragment length polymorphism (RFLP); Mycoplasma gallisepitcum

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