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Differentiation of neuraminidase in N1 subtype from that inN2
subtype of avian influenza A virus
WANG Xiurong1, LI Dongmei2, DENG Guohua1, TIAN
Guobin1,
JIANG Yongping1, LI Chengjun1, LIU Liling1, CHEN
Hualan1
(1. Harbin Veterinary Research Institute, Chinese Academy of Agricultural
Sciences,Harbin 150001, China;
2. College of Veterinary Medicine, Northeast AgriculturalUniversity, Harbin 150030,China ) |
Abstract: Avian influenza viruses (AIV) are divided into different subtypes based on antigenicities of the surface protein hemagglutinin (HA) and neuraminidase (NA), and 15 HA subtypes and 9 NA subtypes have been isolated from birds. At present, AIVs with N1 (H5N1) or N2 (H5N2 and H9N2) NA are widely isolated in poultry. In this study, we developed an RTPCR method to differentiate N1 subtype AIVs from N2 subtype AIVs. The target PCR product was
358bp and 377bp respectively. The reference virus strains serve as positive control, which include A/goose/Guangdong/1/96(H5N1), A/Turkey/England/N28/73(H5N2), A/African starling/983/79(H7N1) and A/Turkey/Wiscosin/1/66（H9N2）. We use this method inspect 37 influenza virus isolates which include 16 N1 subtype viruses and 21 N2 subtype viruses. The PCR positive ratios were 87.5%(14/16) and 95.2%(20/21) respectively. Results showed that the PCR primers have much advantages. In addition, we tested 50 positive samples stochastic, the ratio of veracities was 98.0%(49/50).
Key words: avian influenza virus; neuraminidase; subtype differentiation; RTPCR |
