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蛋鸡血清与卵黄中禽流感病毒抗体的相关性
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黄建文1, 毛跟年2, 马秋明1, 何维明1, 朱旭东1, 王 霞3, 赵 卓1, 李联正1
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(1. 西北农林科技大学 动物科技学院, 陕西 杨凌 712100; 2. 陕西科技大学 生命
科学与工程学院, 陕西 咸阳 712000;3. 陕西咸阳渭城区畜牧兽医站, 陕西 咸阳 712000)
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摘要:
用禽流感(AI)H9N2油乳剂灭活疫苗免疫非免疫蛋鸡,并于免疫后第0、3、5、7、9、11、13、15、17、19、21、30d分别测定血清及卵黄中的AI血凝抑制试验(HI)及琼脂扩散凝集试验(AGP)抗体。结果显示,免疫后第7d卵黄中即可检出HI及AGP抗体,以后逐渐上升,在免疫后第19~21d达高峰值,与血清的HI抗体水平接近,差异不显著( P >0.05)。表明通过对鸡蛋卵黄AIV抗体检测,可以进行AI的疫情监测,但卵黄抗体检测具有一定的滞后性。 关键词:
禽流感; 卵黄抗体; 血清抗体
中图分类号:S 852.659.5 文献标识码:
B 文章编号:1000-6419(2004)05-0065-03 |
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Correlation between avian influenza virus antibody in serum of chickens and that in yolk
HUANG Jianwen1, MAO Gennian2, MA
Qiuming1, HE Weiming1,
ZHU Xudong1,
WANG Xia3, ZHAO Zhuo1, LI
Lianzheng1
(1. College of Animal Science and Technology, Northwest SciTech University of Agricultureand Forestry, Yangling 712100,China;
2. College of Life Science and Engineering, Shaanxi SciTech University, Xianyang 712000,China;
3. Animal Husbandry and Veterinary Station of Weichen District in Xianyang City, Xianyang 712000,China) |
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Abstract: In this study, nonvaccine layers were vaccined with the oil emulsion adjuvant inactive avian influenza(H9N2) vaccine. By means of using the haemagglutination inhibition (HI) test and the agar gel precipitation (AGP) test, antibodies in yolk and serum from the layers vaccined with the vaccine were detected on 0,1,3,5,7,9,11,15,17,19,21 and
30day postvaccination. Result showed that the antibody in yolk can be detected on 7 day postvaccination, from then on, the correlation is more high, and that the top comes on from 19 day to 21 day postvaccination. The mentionedabove result indicated that avian influenza plague can be monitored with the antibody in yolk to avian influenza virus.
Key words: avian influenza; antibody in yolk; antibody in serum |
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