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猪胸膜肺炎放线杆菌PCR检测方法的建立

逯忠新，杨学山，赵卫平，赵萍, 高鹏程， 储岳峰

（中国农业科学院 兰州兽医研究所, 甘肃 兰州 730046）

摘要： 根据猪胸膜肺炎放线杆菌(APP)外膜脂蛋白基因序列，设计合成了1对特异性引物。经PCR扩增，APP 1～10标准血清型菌株均能扩增出大小为980bp的DNA片段，而大肠埃希氏菌、猪多杀性巴氏杆菌、猪链球菌、猪肺炎霉形体和葡萄球菌等的扩增结果均为阴性。该方法检测APP DNA的敏感性可达2pg。表明，此PCR方法特异性好，敏感性高，可用于猪传染性胸膜肺炎的快速诊断。

关键词： 猪胸膜肺炎放线杆菌； PCR； 外膜脂蛋白基因

中图分类号：S 852.619  文献标识码： A  文章编号：1000-6419(2004)06-0006-03

Establishment of PCR method for detection of Actinobacillus pleuropneumoniae

LU Zhong-xin, YANG Xue-shan, ZHAO Wei-ping, ZHAO Ping,GAO Peng-cheng, CHU Yue-feng

(Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046,China)

Abstract: According to nucleic acid sequence of outer membrane lipoprotein gene of Actinobacillus pleuropneumoniae (APP), a pair specific primers were designed. With the primers, DNA fragments 980 bp in length were amplified from 10 serotypes of APP standard strains by PCR. The sensibility of the PCR method is 2 pg. It was concluded from the abovementioned results that the established PCR method can be used to diagnose quickly porcine infectious pleuropneumoniae.

Key words: Actinobacillus pleuropneumoniae(APP); PCR; outer membrane lipoprotein gene

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