| |
|
 |
|
|
|
猪水泡病病毒非结构蛋白编码区的克隆与序列分析
|
|
冶贵生1,2, 刘湘涛1, 张彦明2, 马玉花2, 邓明俊2, 洪海霞2, 韩雪清1
|
|
（1. 中国农业科学院 兰州兽医研究所 农业部畜禽病毒学重点实验室， 甘肃 兰州730046；
2. 西北农林科技大学 动物科技学院， 陕西 杨凌712100）
|
|
摘要：
以猪水泡病病毒（Swine vesicular disease virus,SVDV）HK’70为材料，用特异性引物扩增出非结构蛋白编码区的4个重叠目的片段,连接于pMD18T载体，转化JM109感受态细胞。经对重组质粒双酶切、PCR鉴定、序列分析表明,HK’70非结构蛋白编码区核苷酸长4002nt，氨基酸长1334aa;与J1’73、H/3’76、SVDV(Seechrn等测株)、NET/1/92的核苷酸同源性分别为98.6%、98.3%、98.3%、91.2%，推导氨基酸序列的同源性分别为98.9%、99.1%、99.2%和97.2%。 关键词：
猪水泡病病毒； 非结构蛋白编码区； 序列分析； 同源性
中图分类号：S 852.659.6：Q 52 文献标识码：
A 文章编号：1000-6419(2004)07-0013-06 |
|
|
Cloning and analysis of nucleotide sequences encoding nonstructure
protein of swine vesicular disease virus
YE Guisheng1,2, LIU Xiangtao1, ZHANG Yanming2， MA
Yuhua2,
DENG MingJun2, HONG Haixia2， HAN Xueqing1
(1. Key Laboratory of Animal Virology,Ministry of Agriculture/Lanzhou Veterinary Research Institute,
Chinese Academy of Agricultural Sciences,Lanzhou 730046, China; 2.College of Animal Science and
Technology,Northwest SciTech University of Agriculture and Forestry,Yangling 712100,China) |
Abstract: Four overlapping fragments encoding nonstructure protein of swine vesicular disease virus, which had been amplified from SVDV Hk’70 by 4 pairs of primers, respectively, were ligated with
pMD18T and transformed into Escherichia coli competent cell JM109, respectively. The recombinant plasmids were identified by restriction enzyme and PCR. Homologyanalysis showed that nonstructure protein sequences of SVDV HK’70 shared 98.6%,98.3%,98.3% and 91.2% homology with that of SVDV J1 73,SVDV H/3’76,SVDV(Seechurn) and SVDV NET/1/92, respectively,and the deduced amino acid sequences from nonstructure protein sequences of SVDV HK’70shared 98.9%,99.1%, 99.2% and 97.2% homology with that from nonstructure protein sequenceof SVDVJ1’73,SVDV H/3’76，SVDV(Seechurn) and SVDV NET/1/92, respectively.
Key words: swine vesicular disease virus(SVDV); nonstructure protein sequence; homology |
|
|
|
|
|
|
|
《中国兽医科技》 ©
版权所有
Chinese Journal of Veterinary Science and Technology(CJVST)
webmaster@cjvst.com |
|
