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Display of globular domain of HN protein of Newcastle disease
virus on bacteriophage T4 capsid surface
LIU You, BI Yingzuo, CAO Yongchang, MA Jingyun, XIAO Junfang
(College of Animal Science, South China Agricultural University, Guangzhou 510640,China) |
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Abstract:HN gene with length of 1713bp of Newcastle disease virus was amplified by RTPCR. The PCR product was purified and cloned into pGEMT Easy vector and the recombinant plasmid was sequenced. According to the HN gene sequencing result, another pair of primers containing Eco RⅠrestriction site was designed to amplify the HN fragment coding the globular domain, i.e. the main functional region of HN protein. Then the HN fragment was inserted into the 3′ terminal of SOC gene of pSOC plasmid. The recombinant plasmid pSOCHN was conducted into E.coli BL21(DE3) to induce HN gene expression with
1mmol/L IPTG. A SOCHN fusion protein band with molecular weight of 67ku was dectected on the SDSPAGE gel and nitrocellulose membrane，and the expression products can specifically reacted with the antisera against Newcastle disease virus.
Key words: Newcastle disease virus; HN gene; SOC gene; bacteriophage T4 |
