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DImmune screening of cDNA library from〖WTHX〗 Cysticercus cellulosae
ZONG Ruiqian 1,2, JING Zhizhong2, CAI
Xuepeng2
(1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070,China;
2. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046,China; ) |
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Abstract: The cDNA library from Cysticercus cellulosae had been constructed with the vector pSPORT1. After the library was diluted with the SOC and distributed well on the LB plates(Amp+),the bacteria had been cultured for 14 hours. While the bacteria were grown well and transferred on the nitrocellulose filters(NC) and then put the NC on the LB plates(containing IPTG),the bacteria had been cultured for 4 hours at
37℃.The NC were stifling in chloroform for 15 min and put into lysisbuffer for 14 hours. After the NC were washed and closed, then put into the positive serum. If sheep antipeople IgGHRP and substrate were added, the positive clones would appear.
Key words: Cysticercus cellulosae; cDNA library; immune screening |
