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我国三省区细粒棘球绦虫基因的变异分析

杨俊克1,2， 贾万忠2， 景涛1， 田广孚2， 程晓红2

(1. 兰州医学院 病原生物学教研室， 甘肃 兰州730000；
2. 中国农业科学院 兰州兽医研究所， 甘肃 兰州730046)

摘要： 对从青海省、甘肃省和新疆维吾尔族自治区采集的24株细粒棘球蚴，用线粒体DNA的CO1基因和ND1基因结合rDNA的ITS2测序，调查了不同地区分离株基因型的变异情况。结果显示，所有分离株均为普通羊株（G1基因型）；CO1基因序列的变异率为0～0.8%，ND1基因的变异率为0.1%～0.7%；青海分离株ITS2序列的变异率为0.4%～3.1%；2株青海省西宁市和1株甘肃省武威市分离株分别在CO1基因和ND1基因序列不同位点发生的1处核苷酸非同义替换，导致1个编码的氨基酸替代。研究表明，我国上述3省、区部分区域存在的细粒棘球蚴属于同一株（G1基因型）。

关键词： 细粒棘球绦虫； 基因型； 变异； CO1； ND1； ITS2

中图分类号：R 383.33：Q 754  文献标识码： A  文章编号：1000-6419(2004)09-0012-05

Analysis of genetic variation of Echinococcus granulosus collected 
from three provinces in China

YANG Junke1,2, JIA Wanzhong2, JING Tao1， TIAN GuangFu2， CHENG Xiaohong2

(1. Department of Parasitology, Lanzhou Medical College, Lanzhou 730000, China; 2. Lanzhou 
Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China)

Abstract: Twentyfour Echinococcus granulosus isolates, which were collected from Qinghai Province, Gansu Province and Xinjiang Region in China, were examined with CO1， ND1 and ITS2 sequencing to investigategenotype of the isolates. The results were as follows. All the Echinococcus granulosus isolates were identified to be the common sheep strain (G1 genotype). When nucleotide sequences of both CO1 gene and ND1 gene were compared, variation percentage of 0-0.8% and 0.1%-0.7% existed respectively. Variation percentage of ITS2 of the isolates from Qinghai Province was 0.4%-3.1%. Two isolates from Qinghai Province and one isolate from Gansu Province had nonsynonymous substitations at the differentpositions of CO1 gene and NO1 gene sequences, which resulted in one amino acid replacement substitution. The study indicated that one strain (G1 genotype) existed in parts of Qinghai, Gansu and Xinjiang.

Key words: Echinococcus granulosus; genotype; variation; CO1; ND1; ITS2

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