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动物源食品中残留克伦特罗检测抗体的制备与应用
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胥传来， 贺铁明， 王武康， 彭池芳， 金征宇
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（江南大学 食品学院， 江苏 无锡214036）
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摘要：
采用重氮化法将盐酸克伦特罗(CL)与牛血清白蛋白(BSA)交联，用紫外扫描和凝胶电泳对交联物鉴定后免疫新西兰纯毛白兔，制备抗血清。经鉴定，抗血清中含有针对CL的抗体，效价为3.2×105；以此抗体为基础,设计了间接酶联免疫法,该法的检出限为
0.096ng/mL,对加于尿液、血液和组织中CL的回收率为 80%～120%；通过重复性、灵敏度、准确性等指标对该法进行了评估,并与高效液相层析(HPLC)及国外进口试剂盒进行了比较,表明该法的各项参数均能满足实际检测的需要。 关键词：
克伦特罗； 抗体； 酶免疫检测
中图分类号：S 859.84 文献标识码：
B 文章编号：1000-6419(2004)12-0050-05 |
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Preparation and application of clenbuterol antibody
XU Chuanlai， HE Tieming， WANG Wukang， PENG Chifang, JIN
Zhengyu
(School of Food Science and Technology, Southern Yangtze University ,Wuxi 214036, China) |
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Abstract: Clenbuterol (CL)was coupled with bovine serum albumin by diazotization to synthesize immune antigen. After identification by ultravioletscan andSDS denatured protein gel electrophoresis, the CLBSA conjugate was used to immunize New Zealand rabbit. The antibody against clenbuterol was obtained from the rabbit antiserum. Its titre was 1∶3.2×105. The clenbuterol antibody was used for ELISA kit preparation and it was showed that the minimum detection value was
0.096ng/mL. Some parameters such as reproducibility, sensitivity ,precision, accuracy were evaluated. The results indicate that the kit can be applied to actual detection.
Key words: clenbuterol; antibody; enzymelinked immunoassay |
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