|
Immune test of AI H5 inactivated oil emulsion vaccine in ducks, geese and chickens
CHEN Ruiai1， WEN Zhifen2， TANG
Manhua1，
CHENG Hanbo1， LI Lin1
(1. Guangdong Wen’s Foodstuffs Group Co.,Ltd., Xinxing 527439,China；
2. College of Animal Science,South China Agricultural University, Guangzhou 510642,China) |
|
Abstract: Three week old ducks, and geese,4 week old Wugu chicks and 2 week old Linnan chicks were injected respectively with the AI H5N2 inactivated oil emulsion vaccine.The vaccine was safe and could induce immune protectivity on day from 7 to 10 post vaccination. For ducks and geese,the protective ratio against AIV H5N1 was on day from 9/10 to 10/10, and the antibody reached the highest level,an average titer of
6.2lb-7.0lb, on day 21 post vaccination; on day 120 day post vaccination,the antibody titer was not less than 6.0 lb. The Wugu chicks and Linnan chicks all survived when being challenged by AIV H5N1 on day 14 post vaccination,and had a average antibody titer of
6.4lb-8.1lb, the titer reached the highest level on day 21 post vaccination; on day 90 post vaccination,the antibody titer was not less than
6.2lb. Being vaccinated with a dosage of 0.5mL per duck and 1mL per goose,all of the ducks and geese survived when being challenged by H5N1,and the challenged virus couldn’t be isolated from the duck’s and goose’s cloaca. Being vaccinated with a dosage of 0.2 or
0.3mL per duck and 0.2mL per goose,some of the ducks and geese survived when being challenged by H5N1,and the challenged virus could be isolated.When being vaccinated with a dosage of
0.5mL,the geese all could survive,but the challenged virus still could be isolated. When being vaccinated respectively with a dosage of 0.2,0.3 or
0.5mL per chicken,all of the Wugu chicks and Linnan chicks could survive from being challenged by H5N1,but the challenged virus could be isolated from the chick’s cloaca when the vaccine dosage was
0.2mL per chicken.All of the control groups died from challenging of AIV H5N1,and the virus could be isolated from them.
Key words: avian influenza; H5N2; inactivated vaccine; duck; goose; chicken |
