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传染性腔上囊病病毒VP2基因表达条件的优化
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荣俊 1 ，程太平 2 ，刘晓娜
2 ，刘园 1 ，杨待建 2
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(1. 长江大学 生命科学学院，湖北 荆州434025; 2. 长江大学 动物科学学院，湖北 荆州434025)
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摘要：
用摇瓶培养法，对用作生产传染性腔上囊病基因工程亚单位疫苗的基因工程菌种BL21/pET28a VP2的表达条件进行了优化研究。其结果为：温度37℃，诱导表达时间3～7h，用乳糖诱导时终浓度为20mmol/L时即达到最高表达量；用IPTG诱导时终浓度为0.33mmol/L时即达到最高表达量；乳糖的诱导效果优于IPTG。 |
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关键词：
传染性腔上囊病； 大肠埃希氏菌； 表达条件； 基因工程； 亚单位疫苗 |
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中图分类号：S 855.1：Q 786 文献标识码：
A 文章编号：1000-6419(2005)03-0186-04 |
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Optimization of expressing condition of infectious bursal disease virus VP2 gene in Escherichia coli |
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RONG Jun 1 , CHENG Tai ping
2 , LIU Xiao na 2 , LIU Yuan
1 , YANG Dai jian 2 |
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(1. College of Life Science, Yangtze University, Jingzhou 434025,China; 2. College of Animal Science, Yangtze University, Jingzhou 434025,China) |
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Abstract: Expressing condition of gene engineering strain Escherichia coli BL21/pET28a VP2, which could be used for production of the subunit vaccine against infectious bursal disease, was performed using the method of rocking bottle culture. The result showed that the inducing temperature should be
37℃, the inducing expression time should last 3 to 7 hours, and the inducer could be lactose or isopropyl β D thiogalactopyranosid (IPTG). The maximum expression capacity could be obtained when the concentration of lactose reached
20mmol/L, or the concentration of IPTG reached 33mmol/L. |
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Key words: infectious bursal disease; Escherichia coli; expression condition; gene engineering; subunit vaccine |
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