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抗猪传染性胃肠炎病毒单克隆抗体杂交瘤细胞株的建立
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杨百亮1，冯 力2，赵翠萍1，孙东波2，张建斌
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（1. 天津农学院 动物科学系，天津 300384； 2.中国农业科学院 哈尔滨兽医研究所，黑龙江 哈尔滨 150001）
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摘要：应用杂交瘤技术获得了1株分泌抗猪传染性胃肠炎病毒（TGEV）单克隆抗体（McAb）杂交瘤细胞2D6。经检测，其分泌的抗体亚类为IgG3 ；杂交瘤染色体数为88；间接ELISA检测细 胞培养上清液效价为1∶256，诱生腹水抗体效价达1∶6×10 4 ，与猪流行性腹泻病毒（PEDV）、猪轮状病毒（PRV）、猪致病性大肠埃希氏菌（ Escherichia coli ）以及禽传染性支气管炎病毒（IBV）抗原之间均无交叉反应;ELISA阻断试验表明，TGEV特异性阳性血清对TGEV-McAb有明显的阻断作用，该McAb所识别的抗原是TGEV所特有的抗原决定基。
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关键词：
猪传染性胃肠炎病毒；单克隆抗体；杂交瘤细胞株 |
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中图分类号：S 852.659:Q 813.2 文献标识码：
A 文章编号：1000-6419(2005)12-0979-04 |
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Development of hybridomas secreting monoclonal antibody specific to transmissible gastroenteritis virus of pigs |
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YANG Bai-liang1，FENG Li2，ZHAO
Cui-ping1，SUN Dong-bo2，ZHANG Jian-bin 1
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（1. Department of Animal Science，Tianjin Agricultural College，Tianjin 300384，China；2.Harbin Veterinary Research Institute，Chinese Academy of Agricultural Sciences，Harbin 150001，China）
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Abstract: A hybridoma cell line producing monoclonal antibody against transmissible gastroenteritis virus of pigs was established by the hybridoma technique. The antibody，designated as 2D6，of subclass IgG
3 was isolated. The number of chromosome of the hybridoma cell line was 88. The ELISA titers of as-cite and cell supernatant was 1∶6×10
4 and 1∶256，respectively. No cross-reaction was observed between the McAb and PEDV，PRV，Escherichia coli and IBV. Specific TGEV positive serum inhibited the reac-tion of the McAb with TGEV competitively. The McAb was highly specific to the antigenic determinant of
TGEV. |
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Key words:TGEV；monoclonal antibody；hybridoma cell line |
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