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青海省格尔木市乳牛新孢子虫病的血清学调查
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晁万鼎1，马利青2，李文昌1，张作秀1，李东曲1，韩文阁
1
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（1. 青海省格尔木市畜牧兽医工作站，青海 格尔木 816000； 2.青海省畜牧兽医科学院，青海 西宁 810003）
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摘要：用重组蛋白GST-NcSAG1t作为ELISA诊断抗原，对格尔木市乳牛犬新孢子虫病进行了调查。结果，从 35份乳牛血清样品中检出犬新孢子虫抗体阳性血清4份，阳性率为11.42%。检测结果说明，青海省格尔木市饲养的乳牛牛群中存在犬新孢子虫感染。 |
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关键词：
犬新孢子虫病；重组蛋白；乳牛；血清学调查 |
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中图分类号：S 852.723 文献标识码：
A 文章编号：1000-6419(2005)12-1012-03 |
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Serological investigation of Neospora caninum infection in dairy cows in Geermu County of Qinghai Province |
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CHAO Wan-ding1，MA Li-qing2，LI
Wen-chang1，ZHANG Zuo-xiu1，LI Dong-qu1，HAN Wen-ge 1 |
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（1. Geermu Station of Animal Husbandry and Veterinary Medicine，Geermu 816000，China；2.Qinghai Academy of Animal Science and Veterinary Medicine，Xining 810003，China）
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Abstract: The purified GST-NcSAG1t was used in an enzyme-linked immunosorbent assay for detec-tion of Neospora caninum antibodies in dairy cows in Geermu County of Qinghai Province. Field serum samples collected from dairy cows in the prefecture were examined for the diagnosis of neosporosis by using the ELISA. Result which 4（11.42%） samples of the detected 35 samples were positive for antibodies to N.caninum showed that dairy cows in the prefecture were infected with N.caninum. |
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Key words:neosporosis；NcSAG1；dairy cow；serological investigation |
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