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猪生殖与呼吸综合征病毒福州株 GP3 基因的克隆及序列分析
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魏 宏，林锋强，周伦江，王隆柏，庄向生，张德明
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（福建省农业科学院畜牧兽医研究所，福建 福州 350013）
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摘要：
参考GenBank猪生殖与呼吸综合征（PRRS）病毒CH-1a株（AY032626）GP3基因序列设计合成1对引物，用RT-PCR方法扩增PRRSV福州株GP3基因，并对PCR产物进行了克隆和测序。结果显示GP3基因扩增产物为765 bp。经BLAST分析，该基因的核苷酸序列与CH-1a株、BJ-4株和HB-1株的同源性分别为91.5%，98.6%和91.5%;氨基酸序列的同源性分别为 91.4% ，94.9%和90.9%。 |
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关键词：
PRRSV；GP3基因；克隆；序列分析 |
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中图分类号：S 852.651 文献标识码：
A 文章编号：1000-6419(2005)S1-0021-04 |
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Cloning and sequencing of GP3 gene of PRRSV Fuzhou strain |
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WEI Hong，LIN Feng-qiang，ZHOU Lun-jiang，WANG Long-bai， ZHUANG Xiang-sheng，ZHANG De-ming |
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（Research Institute of Veterinary Medicine and Animal Science，Fujian Academy of Agricultural Sciences，Fuzhou 350013，China） |
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Abstract: Based on nucleotide sequence of porcine reproductive and respiratory syndrome virus（PRRSV） CH-1a strain GP3 gene in Genbank，a pair of primers was designed. The GP3 gene of PRRSV Fuzhou strain was amplified by RT-PCR，then the PCR products were cloned and sequenced. The se-quence analysis showed that the GP3 gene was 765 bp in length. The BLAST results showed that the GP3 gene of Fuzhou strain shared 91.5%，98.6% and 91.5% homology with that of CH-1a，BJ-4 and HB-1 strain respectively at the level of nucleotide acid，and 91.4%，94.9% and 90.9% homology respectively at the level of amino acid. |
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Key words: PRRSV；GP3 gene；cloning；sequence analysis |
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