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鸡传染性贫血病毒在 MSB1 细胞上的生长特性研究
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刘泽文1，徐涤平1，周平华1，杨 峻1，段正赢1，汪宏才1，邵华斌1，山口成夫2
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（1. 湖北省农业科学院 畜牧兽医研究所，湖北 武汉 430209；2. 日本农林水产省家畜卫生试验场，日本Tsukuba）
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摘要：
将鸡传染性贫血病毒（CAV）强毒C364和弱毒C545分别接种MDCC-MSB1细胞，分别于接种后1、24、48、72和96 h取样进行病毒含量测定，并就氨基葡萄糖对CAV在MSB1细胞上生长 的影响进行了研究。结果表明，CAV强、弱毒株接种MSB1细胞后，强毒株增殖速度很快，48 h后病毒含量达到高峰，72 h后开始下降;弱毒株生长速度较强毒株慢，最高效价较强毒株低。用氨基葡萄糖处理后的病毒悬液生长速度减慢，说明氨基葡萄糖对CAV在MSB1细胞上的生长有抑制作用。 |
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关键词：
鸡传染性贫血病毒；MSB1细胞；氨基葡萄糖；生长特性 |
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中图分类号：S 852.659.2 文献标识码：
A 文章编号：1000-6419(2005)S1-0025-02 |
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Study on growing characteristics of CAV in MSB1 cells |
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LIU Ze-wen1，XU
Di-ping1，ZHOU Ping-hua1 ，YANG
Jun1，DUAN Zheng-ying1，WANG
Hong-cai1，SHAO Hua-bing1，S.Yamaguchi2 |
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（1. Institute of Animal and Veterinary Sciences，Hubei Academy of Agricultural Sciences，Wuhan 430209，China；2. National Institute of Animal Health，Ministry of Agriculture，Forestry and Fisheries，Tsukuba，Japan） |
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Abstract: Chicken anemia virus virulent strain C364and low virulent strain C545were inoculated in MDCC-MSB1 cells，virus titre was tested at 1st，24th，48th，72nd and 96th h post-inoculation respectively using the competitive PCR. The influence of glucosamine to CAV in MSB1 cells was also studied. The re-sults indicated that virulent strain C364grew faster post-inoculation，the titre of CAV was the highest at 48th h and descended at 72nd h. The low virulent strain C545grew slowly and the titre of CAV was lower than the virulent strain. CAV propagated slower in MSB1 cells treated with glucosamine. |
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Key words:CAV；MSB1 cells；glucosamine；growing characteristics |
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