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鸡体内新城疫病毒融合蛋白抗原的检测
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陈立功1，郝延刚1，盛祖勋2，万洪全2，吴力力2，陈 露2，王宝安2
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1. 河北农业大学 动物科技学院，河北 保定 071001；2. 扬州大学 兽医学院，江苏 扬州 225009）
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摘要：
用3种基因型（Ⅵg、Ⅶd亚型和Ⅸ型）新城疫病毒（NDV）强毒株分别人工感染25日龄雏鸡，以单克隆抗体介导的免疫组化法检测攻毒鸡体内NDV融合蛋白抗原的分布及定位。结果，在鸡多种组织器官中能检测到病毒抗原;在能检测到病毒抗原的器官中，病毒抗原主要定位于淋巴 细胞、网状细胞、巨噬细胞、肝细胞及各种上皮细胞的胞浆内；NDV对肠相关淋巴组织的亲嗜性优先于附近的上皮。试验结果表明，所用NDV强毒株对试验鸡均为泛嗜性病毒。 |
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关键词：新城疫病毒；融合蛋白；鸡；免疫组化法 |
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中图分类号：S 852.659.5 文献标识码：
A 文章编号：1000-6419(2005)S1-0052-03 |
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Detection of fusion protein antigen of Newcastle disease virus in experimental chickens |
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CHEN Li-gong1，HAO
Yan-gang1，SHENG Zu-xun2，WAN
Hong-quan2，WU Li-li2，CHEN
Lu2，WANG Bao-an2
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（1. College of Animal Science and Technology，Hebei Agricultural University，Baoding 071001，China；2. College of Veterinary Medicine，Yangzhou University，Yangzhou 225009，China）
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Abstract: The distribution of fusion （F） protein antigen of Newcastle disease virus was investigated in experimental chickens. 25-days-old chickens were inoculated with velogenic ND viruses of 3 genotypes （Ⅵg ，Ⅶd and Ⅸ），respectively，through oral，ocular and nasal routes. Localization and distribution of the viral antigen in tissues from the infected chickens were detected by using immunohistochemical （IHC） stai-ning for the detection of NDV F protein. As a result，the viral antigen could be detected frequently in 20 organs and tissues. Specific staining was localized in the cytoplasma of lymphocytes，reticulocytes，macro-phages，hepatocytes as well as various epithelial cells. There was marked preferential viral tropism for the lymphoid aggregates associated with the gastrointestinal （GI） tract rather than for nearby epithelial cells. The result showed that the velogenic ND viruses of 3 genotypes have pantropic tropism for chickens. |
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Key words: Newcastle disease virus；fusion protein；chicken；immunohistochemistry |
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