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肽聚糖对鲫鱼嗜水气单胞菌灭活疫苗
免疫增强效果的研究
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郭玉娟，陈学年
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（肇庆学院 水产科学研究所，广东 肇庆526061）
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摘要：
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为了探讨肽聚糖在鱼类抗嗜水气单胞菌（Aeromonas hydrophila，AH）感染中的免疫佐剂作用，用添加A3α肽聚糖的饲料投喂接种FAH疫苗的彭泽鲫（Carassius auratus var. pengze）；通过测定鲫白细胞吞噬活性、血清和体表黏液溶菌酶活性、抗体效价以及活菌攻毒后的免疫保护率，证明肽聚糖与FAH疫苗联合使用，鲫体表黏液和血清溶菌酶活性、白细胞吞噬活性、抗体效价以及活菌攻毒后的免疫保护率均显著提高，表明A3α肽聚糖是FAH疫苗的良好佐剂，能增强鱼类FAH疫苗免疫应答。 |
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关键词：
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嗜水气单胞菌； 彭泽鲫； 肽聚糖 |
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中图分类号：S 941.42 文献标识码：
A 文章编号：1673-4696(2006)01-0033-04 |
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Enhancement of A3αpeptidoglycan on immune effect of inactivated
vaccine against Aeromonas hydrophila |
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GUO Yujuan, CHEN Xuenian |
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(Research Institute of Fishery Science, Zhaoqing University, Zhaoqing 526061,China) |
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Abstract: In order to study the immune adjuvant effect of A3αpeptidoglycan on fishes infected with Aeromonas hydrophila, Carassius auratus var. pengze was inoculated with formalininactivated A. hydrophila and fed with a diet supplemented with appropriate amount of A3αpeptidoglycan . Immunoenhancement action of A3αpeptidoglycan was observed by determining lysozyme activity, phagocytic activity, agglutinating antibody titer and immunological protection rate after challenge with live A.hydrophila. Results showed that A3αpeptidoglycan could be used as an ideal adjuvant, which enhances immune response of fishes to the vaccine. |
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Key words: Aeromonas hydrophila; Carassius auratus var. pengze; A3αpeptidoglycan |
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