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鸡柔嫩艾美球虫子孢子表面抗原基因λMzp57
在Hela细胞中的表达
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秦睿玲1，2，张西臣1，徐占云2，李建华1，尹继刚1，杨举1
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（1. 吉林大学 畜牧兽医学院，吉林 长春130062；
2. 河北北方学院 动物医学系，河北 张家口075131）
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摘要：
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将已构建的柔嫩艾美球虫(Eimeria tenella)子孢子表面抗原pMDMzp57基因片段定向亚克隆到核酸疫苗载体pVAX1上，构建了真核表达载体pVAXMzp57，酶切鉴定正确后，用脂质体介导的转染法转染Hela细胞，进行了体外表达；并用裸DNA质粒经肌肉接种鸡体内表达。经Westernblotting及RTPCR鉴定，表明该基因在Hela细胞及鸡体内均获得表达，并有一定的反应原性。 |
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关键词：
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柔嫩艾美球虫； 子孢子表面抗原； Hela细胞基因； 表达 |
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中图分类号：S 852.723: Q 786 文献标识码：
A 文章编号：1673-4696(2006)01-0037-03 |
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Expression of sporozoite surface antigen gene λMzp57 of
Eimeria tenella in Hela cells |
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QIN Ruiling1，2, ZHANG Xichen1, XU Zhanyun2, LI Jianhua1, YIN Jigang1, YANG
Ju1 |
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(1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062,China;
2.Department of Animal Medicine, Hebei North University,Zhangjiakou 075131，China） |
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Abstract: The constructed pMDMzp57 plasmid containing the sporozoite surface antigen gene of Eimeria tenella was subcloned into a expression vector pVAX1, and the recombinant plasmid pVAX1Mzp57 was constructed and then expressed in Hela cells. Chickens were immunized intramuscularly with the naked DNA plasmid. Westernblotting analysis and RTPCR showed that the gene was expressed in Hela cells and chicken’s body, and the expressed protein could react with the antiE.tenella sera. |
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Key words: Eimeria tenella; sporozoite surface antigen gene; Hela cell; expression |
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