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莱克多巴胺多克隆抗体的制备

于洪侠1，杨曙明1，朱宇2

（1. 中国农业科学院 农业质量标准与检测技术研究所, 北京100081；
2.四川省农业科学院 分析测试中心，四川 成都610066）

摘要：  
采用多元酸酐法活化盐酸莱克多巴胺，用混合酸酐法将活化的莱克多巴胺与载体蛋白(KLH,BSA)偶联，制备了莱克多巴胺免疫原KLHRac和包被抗原BSARac。以KLHRac免疫新西兰白兔获得了莱克多巴胺多克隆抗体，以BSARac为包被抗原，采用间接ELISA检测抗血清，其效价达到1∶6000，间接竞争ELISA测得抗血清的IC50值约为5ng/mL，其与克伦特罗、沙丁胺醇、特布它林的交叉反应性小于0.01％。
关键词：  
莱克多巴胺； 半抗原； ELISA； 抗体
中图分类号：S 859.84  文献标识码： A  文章编号：1673-4696(2006)01-0062-04

Preparation of antiractopamine polyclonal antibodies

YU Hongxia1，YANG Shuming1，ZHU Yu2

(1. Research Institute of Quality Standards and Testing Technology for AgroFood,Chinese Academy of Agricultural 
Sciences, Beijing 100081，China; 2. Center of Analysis and Detection, Sichuan Academy of 
Agricultural Sciences, Chengdu 610066,China)
Abstract:Ractopamine HCl was activated by the multianhydride method and the activated ractopamine was conjugated to a carrier protein by the mixedanhydride method. The carrier protein was keyhole limpet hemocyanin(KLH) and bovine serum albumin(BSA). The KLH conjugate(KLHRac) was used as immunogen and the BSA conjugate(BSARac) was used as the coating antigen for the development of an ELISA. New Zealand rabbits were immunized with KLHRac. Titer of the polyclonal antiserum detected by the developed indirect ELISA method was more than 6,000.The antiserum IC50 detected by an indirect competitive ELISA was approximately 5 ng/mL. The cross reactivity with clenbuterol,salbutamol and terbutaline was less then 0.01%.

Key words: ractopamine； hapten； ELISA； antibody

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