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日粮核苷酸对机体炎症抗炎症平衡体系的影响

李石营，施用晖，乐国伟

(江南大学 食品学院 食品科学与安全教育部重点实验室，江苏 无锡214036)

摘要：  
为探讨日粮核苷酸对小鼠炎症/抗炎症平衡体系的作用，用CpG DNA和脂多糖(LPS)刺激小鼠构建炎症反应模型，观察分别饲喂无核苷酸（NF）日粮和核苷酸（NT）日粮小鼠的炎性细胞因子IL1、IFNγ、抗炎性细胞因子IL10水平和小肠髓过氧化物酶(MPO)活性变化。结果显示，在刺激后第4h时，NT组小鼠的IL1（P<0.05）、IFNγ（P<0.01）和小肠MPO（P<0.01）明显低于NF组，IL10（P>0.05）高于NF组；刺激后第18h时，NT与NF组的IL1（P<0.01）、IFNγ（P<0.01）、IL10（P>0.05）和MPO（P<0.01）均较刺激后第4h有所降低，NT组的IL1（P>0.05）、IFNγ（P<0.05）和小肠MPO（P<0.05）仍低于NF组，IL10（P>0.05）仍高于NF组。表明，日粮核苷酸对CpG DNA或LPS所诱导的炎症反应有明显的抑制作用，有助于维持机体炎症/抗炎症平衡，保护机体免受炎症损伤。
关键词：  
核苷酸； CpG DNA； LPS； 炎症； 抗炎症； 平衡
中图分类号：S 816.7  文献标识码： A  文章编号：1673-4696(2006)01-0066-04

Effects of dietary nucleotides on inflammatory 
and antiinflammatory balance

LI Shiying, SHI Yonghui, LE Guowei

(Key Laboratory of Food Science and Safety, Ministry of Education/ Southern Yangtze University, Wuxi 214036，China)
Abstract: The aim of this study was to evaluate effect of dietary nucleotides on inflammation and antiinflammation homeostasis. Mice were administrated with nucleotidefree(NF) or nucleotidesupplemented(NT) diet, and inflammatory cytokine IL1,IFNγ, antiinflammatory cytokine IL10 and intestinal myeloperoxidase(MPO) were determined at 4th and 18th h after stimulation with CpG DNA and LPS. Production of IL1(P<0.05）,IFNγ（P<0.01） and activity of MPO（P<0.01） decreased, whereas production of IL10（P>0.05）increased with nucleotides supplement at 4th h after stimulation. At 18th h after stimulation, production of IL1（P<0.01）, IFNγ（P<0.01）, IL10（P>0.05） and activity of MPO（P<0.01） were lower than that at 4th h. Production of IL1（P<0.05）,IFNγ（P<0.01） and activity of MPO（P<0.01） in Groups NT were still lower than that in Groups NF, and production of IL10（P>0.05） was still higher than that in Groups NF. The results indicated dietary nucleotides can depress inflammation induced by CpG DNA or LPS, maintain inflammatory and antiinflammatory balance and protect animals from injury.

Key words: nucleotides; CpG DNA; LPS; inflammation; antiinflammation; balance

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