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RNA干扰技术及其在寄生线虫基因
功能研究中的应用
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邹丰才，宋慧群，陈宁，朱兴全
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（华南农业大学 兽医学院，广东 广州510642）
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摘要：
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绍了RNA干扰(RNAi)的概念、线虫中RNAi的作用机理、RNAi的三个分子特性，即可遗传性、转移性RNAi的放大效应和RNAi信号的传播特性。从模板dsRNA的获取、dsRNA的导入和线虫中RNAi效应的检测方法三个方面概述了应用RNAi技术研究线虫基因功能的试验方法和最新进展。 |
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关键词：
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RNA干扰； 线虫； 基因功能 |
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中图分类号：S 852.731 文献标识码：
A 文章编号：1673-4696(2006)01-0080-05 |
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RNA interference technique and its application to studies of gene
functions of parasitic nematodes |
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ZOU Fengcai, SONG Huiqun, CHEN Ning, ZHU Xingquan |
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(College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642,China) |
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Abstract: Concept, mechanism, heritability, transitivity and spreading characteristics of RNA interference in parasitic nematode were introduced. Experimental methods for RNAi in parasitic nematode, including acquirement of dsRNA, introduction of dsRNA and detection of RNAi effect in nematode, and new developments in studies of gene functions of parasitic nematode were also reviewed. |
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Key words: RNA interference; parasitic nematode; gene function |
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