|
|
|
PrP基因在金黄地鼠淋巴和外周组织中
表达水平的动态检测
|
|
|
|
宁章勇1，2， 赵德明2， 杨建民2， 于博1， 孔小明1
|
|
|
|
(1. 华南农业大学 兽医学院， 广东 广州510642；
2. 中国农业大学 动物医学院 国家动物传染性海绵状脑病实验室， 北京100094)
|
|
|
|
摘要：
|
|
利用实时荧光定量RT-PCR技术，构建了标准重组质粒，制备了标准曲线，对不同年龄金黄地鼠腹股沟浅淋巴结、脾、心、肝、肺和肾提取总RNA，反转录后进行PrP基因的表达定量。结果发现，淋巴组织呈现高的表达量，外周组织的表达量比较低；不同组织在不同年龄出现表达高峰。 |
|
关键词：
|
|
金黄地鼠； 淋巴组织； 外周组织； 朊蛋白； 基因表达 |
|
中图分类号：S 852．659．7 文献标识码：
A 文章编号：1673-4696(2006)03-0177-06 |
|
|
Dynamic detection of expression level of prion gene in lymphatic
tissue and peripheral organs of Mesocricetus auratus |
|
|
|
NING Zhang-yong1，2， ZHAO
De-ming2， YANG Jian-min2， YU
Bo1, KONG Xiao-ming1 |
|
|
|
(1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642,China;
2. National Laboratory of Animal Transmissible Spongiform Encephalopathies/College of
Veterinary Medicine, China Agricultural University, Beijing 100094, China) |
|
|
Abstract: The standard plasmid and the standard curve were constructed to quantify the PrP gene expression by using the
real-time fluorescent RT-PCR technique.Total RNA was isolated from spleen, inguinal shallow lymphoid node, heart, liver, lung and kidney of mice at different ages to quantify the gene expression after the reverse transcription. Results showed that high mRNA levels were present in the lymphatic tissue but low in peripheral organs. The expression peaks of the prion gene were different for the different tissues. |
|
|
|
Key words: Mesocricetus auratus; lymphatic tissue; peripheral organ; prion protein; gene expression |
|
|
|
|
|
《中国兽医科学》 ©
版权所有
Chinese Veterinary Science
zgsykx@zgsykx.com |
