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三种抗原对家畜日本血吸虫病诊断价值的比较
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彭运潮1，2，章登吉2，4，石耀军2，李浩2，陆珂2，刘金明2，
孙安国2，刘增再1，李海辉1，胡述光3，林矫矫2
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(1. 湖南农业大学 动物科技学院，湖南 长沙410128； 2. 中国农业科学院 上海家畜寄生虫病研究所，
上海200232； 3. 湖南生物机电职业技术学院， 湖南 长沙410128；
4. 上海师范大学 生命与环境学院， 上海200232)
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摘要：
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以日本血吸虫基因重组抗原LHD-Sj23/pGEX、可溶性虫卵抗原（soluble egg antigen, SEA）及SEA经Sephadex
G-200柱层析分离后得到的第一峰SEA1作为诊断抗原，应用ELISA检测人工感染日本血吸虫绵羊血清及自然感染日本血吸虫水牛血清。结果显示，三种抗原用于检测人工感染日本血吸虫绵羊血清的阳性符合率分别为88.79%、98.15%、100%，阴性符合率均为100%；自然感染日本血吸虫水牛血清的阳性符合率分别为80%、80%、84%，阴性符合率分别为88.9%、93.3%、91.1%；三种抗原与伊氏锥虫病牛血清无交叉反应；检测感染日本血吸虫不同时间绵羊血清，发现感染6周后均被检出针对这三种抗原的特异性抗体，且SEA及SEA1在感染4周后即可检出特异性抗体。三种抗原的诊断效果差异不显著。 |
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关键词：
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日本血吸虫； 抗原； 诊断； 绵羊； 水牛 |
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中图分类号：S 852．735 文献标识码：
A 文章编号：1673-4696(2006)03-0207-05 |
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Comparison among three antigens for the diagnosis of
schistosomiasis japonicum in domestic animals |
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PENG Yun-chao1，2, ZHANG
Deng-ji2，4， SHI Yao-jun2， LI
Hao2， LU Ke2,
LIU Jin-ming2, SUN An-guo2, LIU
Zeng-zai1, LI Hai-hui1, HU Shu-guang3, LIN
Jiao-jiao2 |
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(1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2. Shanghai
Institute of Domestic Animal Parasitology, Chinese Academy of Agricultural Sciences, Shanghai 200232, China;
3. Hunan Biological and Electromechanical Polytechnic College, Changsha 410128, China;
4. College of Life and Environment, Shanghai Normal University,Shanghai 200232, China) |
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Abstract: The fusion protein LHD-Sj23/pGEX, the soluble egg antigen(SEA) and the gel
chromatography-purified soluble egg antigen（SEA1）were employed as antigens in ELISA to detect serum samples from experimentally infected sheep and naturally infected buffalo with Schistosoma japonica . Results showed that 88.79%, 98.15% ,100% and 80%,80%,84% positive coincidence rates, 100%,100%,100% and 88.9%,93.3%,91.1% negative coincidence rates were obtained, respectively. No
cross-reaction was found in 18 serum samples from cattle with trypanosomosis. The dynamics of the specific antibody in
se-rum of sheep infected with S.japonicum was examined on weeks 2, 4 and 6. Specific antibody was detected on week 6 in serum from all infected sheep using each of the three antigens. Specific antibody was also detected on week 4 in serum of some of the infected sheep using SEA or SEA1. Statistical analysis using the
two-sample-t-test demonstrated that there was no significant difference in the detection rates among the three antigens. |
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Key words: Schistosoma japonicum; antigen; diagnosis; sheep; buffalo |
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