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鹅胰岛素样生长因子-Ⅰ基因的克隆及其
mRNA在组织中的表达量变化
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肖翠红1，李馨1，佟春玉1，颜国华1，刘国君2
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（1.黑龙江八一农垦大学 动物科技学院，黑龙江 大庆163319；
2.黑龙江省鹅育种中心,黑龙江 齐齐哈尔160015）
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摘要：
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利用半定量反转录聚合酶链反应(RT-PCR）克隆了黑龙江籽鹅IGF-Ⅰ基因，并检测了生长期间该基因mRNA在各组织中的表达量变化。结果表明，鹅IGF-Ⅰ基因与鸡、鸭IGF-Ⅰ基因的同源性分别为97.6%、99.3%；鹅肝组织中IGF-Ⅰ mRNA的表达水平在30日龄和60日龄较高，而30日龄的表达量极显著高于22、28日龄鹅胚和1日龄籽鹅，显著高于10日龄雏鹅；30日龄雏鹅IGF-ⅠmRNA在肝、卵巢、胸肌、肺、脾、腿肌、垂体、肌胃、肾、心等组织中都有表达，其中肝、卵巢、胸肌、肺的表达量较高。 |
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关键词：
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鹅； 胰岛素样生长因子-Ⅰ； 半定量反转录聚合酶链反应 |
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中图分类号：S 858.33:Q 786 文献标识码：
A 文章编号：1673-4696(2006)03-0216-04 |
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Cloning of insulin-like growth factors-Ⅰ gene from
goose and its expression dynamics in tissues |
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XIAO Cui-hong1, LI
Xin1, TONG Chun-yu1, YAN
Guo-hua1, LIU Guo-jun2 |
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(1.College of Animal Science and Technology ,Heilongjiang August First Land Reclamation University,
Daqing 163319, China; 2.Heilongjiang Geese Breeding Center, Qiqihaer 160015,China) |
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Abstract: An insulin-like growth factors-Ⅰ gene(IGF-Ⅰ) from geese was cloned and its expression dynamics in tissues was examined by
semi-quantitive RT-PCR using β- actin as an internal standard. The sequence similarities in
IGF-Ⅰgene between goose and chicken, and goose and duck were 97.6% and 993%, respectively. The expression of
IGF-Ⅰin liver was more abundant on days 30 and 60 than on the other investigated days, and the expression of IGFⅠmRNA reached the highest on day 30. A positive correlation was established between expression of
IGF-Ⅰ mRNA and weight gain. The IGF-Ⅰ mRNA was expressed in liver, ovary，breast，lung，spleen，leg muscle，pituitary，stomach，kidney and heart, and higher abundance of
IGF-Ⅰ mRNA was found in liver，ovary，breast and lung. |
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Key words: goose； IGF-Ⅰ； semi-quantitive RT-PCR |
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