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鸡病毒性关节炎病毒C-98分离株S1基因
的克隆与序列分析
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常继涛1，关平原1，乌日罕2，马立峰2，于富丽2,特木尔巴根2
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(1.内蒙古农业大学 动物科学与医学学院，内蒙古 呼和浩特010018；
2.内蒙古动物疫病预防与控制中心，内蒙古 呼和浩特010010)
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摘要：
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提取鸡病毒性关节炎病毒(AVAV)内蒙古分离株（C-98株）总RNA，参考GenBank中禽呼肠孤病毒(ARV)S1133株S1基因序列设计了2对引物，应用RT-PCR技术扩增了病毒S1基因，将S1基因cDNA克隆到pGEM-T Easy载体后测序。将测定序列拼接后与参考毒株ARV-176、 ARV-138、 ARV-S1133、MDRV-YJL的S1基因序列进行了比较。结果显示，AVAV C-98分离株的S1基因与强毒株ARV-176株的同源性最高，达999%；与MDRV-YJL的同源性为993%，与弱毒疫苗株ARV-S1133的同源性也达97.9%；与ARV-138株的同源性最低，为812%。表明，AVAV C-98株与参考毒株的差异较小，与疫苗株ARV-S1133有很高的同源性。 |
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关键词：
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鸡病毒性关节炎病毒；S1基因；RT-PCR；序列分析 |
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中图分类号：S 852.659.4：Q 785 文献标识码：
A 文章编号：1673-4696(2006)04-0287-05 |
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Cloning and sequence analysis of S1 gene of
avian viral arthritis virus C-98 strain |
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CHANG Ji-tao1, GUAN Ping-yuan1,
Wurihan2, MA Li-feng2, YU Fu-li2,
Temuerbagen2 |
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( 1.College of Animal Science and Veterinary Medicine, Nei Monggol Agricultural University,Hohhot 010018, China;
2.Center for Prevention and Control of Animal Diseases of Nei Monggol, Hohhot 010010, China) |
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Abstract: Total RNA of avian viral arthritis virus C-98 strain(AVAV C-98) isolated in Nei Monggol, China was extracted. According to the published S1 gene sequence of ARV-S1133 strain in GenBank, two pairs of primers were designed. A S1 gene of AVAV C-98 was amplified by RT-PCR and then cloned into pGEM-T Easy plasmids and then sequenced.The acquired nucleotide sequences were analyzed using computer softwares, and compared with S1 gene sequences of ARV-176, ARV-138, ARV-S1133 and Muscovy duck reovirus strain YJL（MDRV-YJL）.Nucleotide similarities between the S1 gene of AVAV C-98 and that of ARV-176, MDRV-YJL, ARV-S1133 and ARV-138 were 99.9%, 99.3%, 97.9% and 81.2%, respectively. These results indicated that there was little variation between AVAV C-98 and the reference reovirus strains,especially ARV-S1133. |
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Key words: avian viral arthritis virus; S1 gene; RT-PCR ; sequence analysis |
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