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用甘肃棘豆草粉饲喂苦马豆素-BSA免疫
山羊的血清相关酶分析
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童德文，董强，赵宝玉，付广建，张瑾
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（西北农林科技大学 动物科技学院， 陕西 杨凌712100）
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摘要：
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用甘肃棘豆草粉饲喂经苦马豆素（SW）-BSA免疫接种的山羊，检测分析了山羊血清中与中毒相关的酶的活性，以探讨SW-BSA免疫接种对动物机体组织器官的保护作用。将24只山羊随机分为免疫对照组、免疫攻毒组和未免疫攻毒对照组，免疫对照组和免疫攻毒组山羊接种SW-BSA，免疫攻毒组和未免疫攻毒对照组山羊按干重拌料饲喂10g/(kg·d)甘肃棘豆草粉，检测血清谷草转氨酶（GOT）、谷丙转氨酶（GPT）、乳酸脱氢酶（LDH）、碱性磷酸酶（AKP）、尿素氮（BUN）和α-甘露糖苷酶（AMA）的变化。结果表明，免疫攻毒组山羊较未免疫攻毒对照组山羊LDH活性升高延缓28d，AKP活性升高延缓14d，AMA活性降低延缓21d，BUN活性升高延缓14d，GOT活性升高延缓28d。这些酶活性延缓变化，说明SW-BSA免疫动物后，在甘肃棘豆攻毒后的30d内，能够有效地延缓SW对山羊肝、心和肾等组织器官的损伤。 |
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关键词：
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苦马豆素-BSA; 血清酶; 甘肃棘豆; 山羊 |
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中图分类号：S 852.4 文献标识码：
A 文章编号：1673-4696(2006)04-0331-05 |
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Analysis of serum enzyme of goats immunized with SW-BSA after
poisoning with Oxytropis kansuensis |
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TONG De-wen, DONG Qiang, ZHAO Bao-yu，FU Guang-jian, ZHANG Jin |
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(College of Animal Science and Technology, Northwest Sci-Tech University of
Agriculture and Forestry, Yangling 712100, China) |
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Abstract: 24 goats were divided into immune control group, immune and poisoning group, poisoning control group randomly. Goats in the immune control group and immune poisoning group were vaccinated with SW-BSA. Goats in the immune and poisoning group, and the poisoning control group were fed with 10g/kg BW/d powder of O. kansuensis each day. The changes of serum GOT, GPT, LDH, AKP, BUN and AMA were examined. Results revealed that the increasing activity of LDH and GOT was delayed for 28 days, the increasing activity of AKP and BUN was delayed for 14 days, and the decreasing activity of AMA was delayed for 21 days in the immunized and poisoning goats compared with poisoning control goats. The results indicated that in 30 days, SW-BSA could prevent liver, kidney and heart of goats from injuring by SW effectively. |
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Key words: SW-BSA; serum enzyme; Oxytropis kansuensis; goat |
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