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H5亚型禽流感病毒HA1基因在Sf9昆虫细胞中的
表达及其抗原性检测
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齐岩，孙凌霜，王彬，孙进华，王君伟，师东方
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（东北农业大学 动物医学院，黑龙江 哈尔滨150030）
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摘要：
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利用pBlueBacHis2杆状病毒载体在昆虫细胞Sf9中表达H5亚型禽流感病毒(AIV)的HA1基因。Western-blotting证明HA1在昆虫细胞中得到了表达，其产物具有抗原性；且不与H7、H9 AIV的抗血清发生交叉反应，具有良好的特异性。Dot-ELISA结果显示，表达蛋白可以在非变性条件下与相应抗体作用，同样具有良好的抗原性。试验结果证明，表达的H5亚型AIV的HA1蛋白，为检测禽类体内是否感染H5亚型AIV和监测免疫禽类血清H5抗体水平提供了优良的检测抗原。 |
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关键词：
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禽流感病毒；HA1基因；杆状病毒；真核表达 |
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中图分类号：S 852.659.5： Q 786 文献标识码：
A 文章编号：1673-4696(2006)05-0352-05 |
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Eukaryotic expression of HA1 gene of H5 avian influenza virus
in insect cells and the antigenicity of the expressed protein |
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QI Yan, SUN Ling-shuang, WANG Bin, SUN Jin-hua, WANG Jun-wei, SHI Dong-fang |
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(College of Veterinary Medicine,Northeast Agricultural University，Harbin 150030，China) |
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Abstract: HA1 gene of H5 avian influenza virus(AIV) was expressed in insect cells by pBlueBacHis2 baculovirus vector. Western-blotting analysis showed that the expressed HA1 protein had antigenicity and did not react with H7 and H9 AIVs anti-serum. Dot-ELISA analysis showed that the expressed recombinant protein had good and special characteristics under non-denaturing conditions. These results demonstrated that the expressed HA1 protein of H5 AIV was a good antigen for detection of H5 AIV infection and for determination of antibody level in vaccinated avian. |
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Key words: avian influenza virus；HA1 gene；baculovirus；eukaryotic expression |
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