|
|
|
口蹄疫A型灭活疫苗毒种和种子批的研究
|
|
|
|
王永录1,2 ，张永光2，方玉珍2，蒋守田2，潘丽2，刘力宽2，吕建亮2，张维德2
|
|
|
|
（1．甘肃农业大学 动物医学院，甘肃 兰州730070；2．中国农业科学院 兰州兽医研究所 家畜疫病病原
生物学国家重点实验室 农业部畜禽病毒学重点开放实验室 国家口蹄疫参考实验室，甘肃 兰州730046）
|
|
|
|
摘要：
|
|
为了保证制苗及攻毒用毒种的质量和稳定性，在对制苗和攻毒毒种的毒力、特异性、保存期、扩繁代次等进行研究的基础上，建立了口蹄疫A型灭活疫苗制苗和攻毒用毒种种子批。结果表明，原始毒种和基础毒种的扩繁代次均控制在5代以内，适宜的保存方法和保存期均为加含500mL/L甘油的PBS后在-30℃保存1年、-70℃保存2年；用于生产抗原的工作毒种，最高扩繁代次控制在15代以内，最佳保存方法和保存期为加保护剂后在-20℃保存半年、-70℃保存1年；用于效力检验的攻毒毒种采用原始毒种。通过制苗和攻毒用毒种种子批的建立，规范了口蹄疫疫苗生产和检验过程中的毒种管理。 |
|
关键词：
|
|
口蹄疫；A型；灭活疫苗；毒种；种子批 |
|
中图分类号：S 852.659.6 文献标识码：
A 文章编号：1673-4696(2006)05-0371-05 |
|
|
Studies on the virus seeds and seed batches of inactivated
vaccine against foot-and-mouth disease virus type A |
|
|
|
WANG Yong-lu1,2, ZHANG Yong-guang2, FANG
Yu-zhen2,JIANG Shou-tian2,
PAN Li2,LIU Li-kuan2, L Jian-liang2, ZHANG
Wei-de2 |
|
|
|
（1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;
2.National Foot-and-Mouth Disease Reference Laboratory/Key Laboratory of Animal Virology， Ministry
of Agriculture/State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,
Chinese Academy of Agricultural Sciences, Lanzhou 730046,China） |
|
|
Abstract: Based on studies on virulence, specificity, storage life and passage of foot-and-mouth disease virus (FMDV) type A seeds, the virus seed batches for vaccine manufacturing and challenge of the inactivated vaccine against FMDV type A were established in order to ensure quality and stabilization of the vaccine. The passages of master and foundation virus seeds were less than 5, and their storage life was 1 year at -30℃ or 2 years at -70℃ in PBS containing 500mL/L of glycerin. The highest passage of producing virus seeds was less than 15, and their storage life was 6 months at -20℃ or 1 year at -70℃. The master virus seed was adopted for challenge. The management of virus seed in the production and verification process was standardized through the establishment of seed batches. |
|
|
|
Key words: foot-and-mouth disease; type A; inactivated vaccine; virus seed; virus seed batches |
|
|
|
|
|
《中国兽医科学》 ©
版权所有
Chinese Veterinary Science
zgsykx@zgsykx.com |
