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重庆市山羊博尔纳病病毒P24基因的检测
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赵立波,谢鹏,牟君,李亚军,张小东,邹德智,刘庆军
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(重庆医科大学 附属第一医院神经内科 重庆市神经病学重点实验室,重庆400016)
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摘要：
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为了解重庆市山羊博尔纳病隐性带毒情况,分析博尔纳病病毒(BDV)的种系来源。采用巢式逆转录酶PCR结合荧光定量PCR(FQ-nRT-PCR)技术对重庆市60只山羊外周血单核细胞(PBMCs)及脑组织中的BDV P24基因片段进行了检测，将阳性产物测序,并与国外BDV毒株进行了比较。结果，山羊外周血检测阳性率为8.3%(5/60)，脑组织检测阳性率为10%(6/60)。该BDV P24片段核苷酸序列与马源BDV H1766株同源性最高，达96.51%，与标准株Strain V和He/80同源性为95.35%，并且编码的氨基酸序列也相同。表明，重庆市山羊中存在动物源性博尔纳病隐性带毒，该BDV P24核苷酸序列与Strain V和He/80株具有高度同源性。 |
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关键词：
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博尔纳病病毒；P24 基因；荧光定量PCR；山羊 |
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中图分类号：S 852.659.5 文献标识码：
A 文章编号：1673-4696(2006)06-0460-04 |
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Detection of Borna disease virus P24 gene in goats in Chongqing |
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ZHAO Li-bo, XIE Peng,MU Jun, LI Ya-jun, ZHANG Xiao-dong, ZOU De-zhi, LIU Qing-jun |
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（Department of Neurology, The First Affiliated Hospital of Chongqing University of
Medical Sciences,Chongqing 400016,China） |
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Abstract: To investigate the recessive infection of Borna disease virus in goats in Chongqing，analyze their origin，and explore if the nucleotide sequence and amino acid sequence in BDV P24 were homophylic with standard strain V and strain He/80. BDV P24 fragment was amplified by nested reverse transcriptase polymerase chain reaction with fluorescence quantitative PCR(FQ-nRT-PCR) in peripheral blood mononuclear cells(PBMCs) and brain tissues from 60 goats.For those positive products, the gene sequence and amino acid sequence were then analyzed and compared with those of the overseas strains detected in human and animals.The positive rate(5/60,8.3%) in PBMCs were higher than that(6/60,10%) in brain tissues. The gene sequence for positive products showed that BDV P24 in goats from Chongqing was highly similar with that of H1766 strain detected from diseased horses(96.51%)and also with that of the standard strain V and strain He/80(95.35%).However, their amino acid sequences remained the same.These results indicated that BDV recessive infection occurred in goats of Chongqing,which probably originated from animals.The gene sequence was significantly similar with that of standard stain V and stain He/80. |
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Key words: Borna disease virus; P24 gene;fluorescence quantitative PCR; goat |
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