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PCV2感染对猪MCP-1和IL-8 mRNA表达的影响
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周双海，杨汉春，郭 鑫，司兴奎，赵东升
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（中国农业大学 动物医学院 农业部预防兽医学重点实验室，北京100094）
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摘要：
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将猪圆环病毒2型（PCV2）BF株经口、鼻接种40日龄健康普通仔猪，于接种后不同时间宰杀，收集肺泡巨噬细胞（PAM），同时设立对照。用竞争PCR技术测定趋化性细胞因子IL-8和MCP-1 mRNA水平，分析PCV2感染对其mRNA表达的影响。结果显示，与对照组相比，在PCV2感染后第7d，IL-8 mRNA水平下降至最低，随后迅速上升，至第14d时达到高峰，而后快速下降，但仍维持较高水平；MCP-1 mRNA水平在攻毒后第3d下降至最低，之后逐渐上升，至第14d时达到高峰，而后下降，第21d以后恢复正常。结果表明，PCV2感染初期可导致PAM趋化性细胞因子IL-8和MCP-1基因的转录明显下调。 |
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关键词：
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猪圆环病毒2型；肺泡巨噬细胞；IL-8；MCP-1；竞争PCR |
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中图分类号：S 852.659.2
文献标识码：
A 文章编号：1673-4696(2006)06-0468-04 |
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Effect of porcine circovirus type 2 infection on porcine
mRNA expression of IL-8 and MCP-1 |
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ZHOU Shuang-hai, YANG Han-chun, GUO Xin, SI Xing-kui, ZHAO Dong-sheng |
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(Key Laboratory for Preventive Veterinary Medicine, Ministry of Agriculture/
College of Veterinary Medicine, China Agricultural University, Beijing 100094, China) |
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Abstract: To explore the effects of porcine circovirus type 2(PCV2) infection on the mRNA expression of chemokine interleukin-8 (IL-8) and monocyte chemotactic protein-1(MCP-1), porcine alveolar macrophages(PAMs) were collected on different day post inoculation(PI) from forty-day-old healthy conventio-nal piglets inoculated oro-nasally with PCV2 BF strain, the mRNA level of IL-8 and MCP-1 were examined by competitive PCR assay. Results showed that the IL-8 mRNA level declined to the neap on 7 day PI, ascended fast and peaked on 14 day PI, then descended but still kept high relatively. The MCP-1 mRNA level declined to the neap on 3 day PI, ascended and peaked on 14 day PI, then descended and resumed to the normal level on 21 day PI. Results of the present study indicated that PCV2 infection could cause the down-regulation of the gene transcription of chemokine IL-8 and MCP-1 in PAMs during early infection. |
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Key words: porcine circovirus type 2; alveolar macrophage; IL-8; MCP-1; competitive PCR |
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