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柔嫩艾美球虫地克珠利和马杜拉霉素抗药株与敏感
株孢子化卵囊18S rDNA基因的差异
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蔡兰1，2，黄兵2，肖明1，韩红玉2，姜连连2，赵其平2，董辉2
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(1.上海师范大学 生命与环境科学学院，上海200234；
2.中国农业科学院 上海兽医研究所 农业部动物寄生虫学重点开放实验室，上海200232)
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摘要：
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为探讨抗球虫药物地克珠利和马杜拉霉素对柔嫩艾美球虫18S rDNA的影响，分别对人工诱导的柔嫩艾美球虫地克珠利抗药株（ETAD）、马杜拉霉素抗药株（ETAM）和药物敏感株（ETDS）孢子化卵囊的18S rDNA基因进行了克隆测序，并通过生物信息软件进行对比差异分析。结果显示，ETAM株有3个碱基发生突变(T170突变为C，T646突变为C，G694突变为C)；ETAD株有1个碱基发生突变(T646突变为C)。经进一步分析比较，发现ETAM株 18S rRNA的二级结构与ETDS株的差异很大，而ETAD株18S rRNA的二级结构则与ETDS株的完全相同。这些碱基的突变有可能是导致E. tenella产生抗药性的原因之一。 |
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关键词：
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艾美球虫；马杜拉霉素；地克珠利；抗药性；18S rDNA |
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中图分类号：S 852.723 文献标识码：
A 文章编号：1673-4696(2006)06-0472-06 |
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Comparison of 18S rDNA sequence of sporulated oocysts between
Eimeria tenella drug-sensitive strain, the anti-diclazuril
and anti-maduramycin strains |
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CAI Lan1,2, HUANG
Bing2, XIAO Ming1, HAN
Hong-yu2,
JIANG Lian-lian2, ZHAO Qi-ping2,DONG
Hui2 |
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(1.College of Life and Environmental Sciences,Shanghai Normal University，Shanghai,200234，China;
2.Key Laboratory for Animal Parasitology, Ministry of Agriculture/Shanghai Veterinary Research Institute,
Chinese Academy of Agricultural Sciences，Shanghai 200232, China) |
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Abstract: To analyze effect of diclazuril and maduramycin on 18S rDNA of Eimeria tenella, the 18S rDNA of sporulated oocyst of the E. tenella anti-diclazuril strain(ETAD), the E. tenellaanti-maduramycin strain (ETAM) and the E. tenella drug-sensitive strain (ETDS ) were cloned and sequenced, and were compared by using DNAStar 5.0 and RNADraw 1.1, respectively. Comparison using the DNAStar 5.0 showed that three mutations, T170→C，T646→C and G694→C, were found in ETAM and one mutation,T646→C, was found in ETAD. Comparison with the RNADraw 1.1 showed that ETAD had the same secondary structure as ETDS, but there was difference in the secondary structures between ETAM and ETDS. These mutations may be related with drug resistance in the E. tenella strains. |
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Key words: Eimeria; maduramycin; diclazuril; drug-resistance; 18S rDNA |
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