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PCR技术在鸟类性别鉴定上的应用
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陈武1，谢淑敏2 ，谢高基1，黄勉1，陈绚姣1，廖明2
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(1.广州动物园，广东 广州510070;
2.华南农业大学 兽医学院 农业部养禽与禽病防治重点开放实验室，广东 广州510642)
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摘要：
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根据鸟类性染色体连锁基因EE0.6在Z、W染色体上长度的不同，通过选择6对不同的引物,同时设置4种不同的反应条件，分别采用PCR技术对丹顶鹤、黑鹳、琉璃金刚鹦鹉、绯红金刚鹦鹉、鞭苔鵎鵼、戴冕鹤、火烈鸟的Z、W染色体上的EE0.6基因进行了特异性扩增。结果显示，某些引物组合能从雄鸟样品中扩增出1条片段（EE0.6Z，190bp），而雌性鸟样品则能扩增出2条片段（EE06W和EE06Z,分别为250bp和190bp），但4组引物对6种鸟类EE0.6基因的扩增效果不同。证实，利用PCR技术能对鸟类进行性别鉴定。 |
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关键词：
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PCR技术；鸟类；性别鉴定；EE0.6基因 |
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中图分类号：Q 324：Q503 文献标识码：
A 文章编号：1673-4696(2006)06-0485-04 |
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Application of PCR technique in sexing birds |
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CHEN Wu1，XIE
Shu-min2，XIE Gao-ji1，HUANG
Mian1，CHEN Xun-jiao1，LIAO
Ming2 |
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(1.Guangzhou Zoo,Guangzhou 510070，China; 2. Ministry of Agriculture/Key Laboratory of Avian Science and Control
of Avian Diseases College of Veterinary Medicine,South China Agricultural University, Guangzhou 510642，China) |
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Abstract:Using the discrepancy between length of the sex-specific EE0.6 gene on the Z and W sex chromosomes of avian species, 6 different pairs of primers and 4 different reaction conditions were used to specifically amplify the EE0.6 gene on the Z and W chromosomes of Grus japonensis, Ciconia nigra, Ara ararauna, Ara macao, Ramphastos toco，Balearica pavonina, and Phoenicopterus ruberer by PCR. Under certain reation conditions, male specimens displayed one distinct amplification band (EE0.6Z,190bp), whereas two bands(EE0.6W and EE0.6Z,250bp and 190bp) were amplified for the female specimens. However, amplifications in 4 different reaction conditions were different in 6 species of birds. It was concluded that the PCR technique is useful for sexing birds. |
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Key words: PCR technique; bird; sexing; EE0.6 gene |
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