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水牛卵母细胞去核方法的比较
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陈自洪，杨素芳，谢英，李日聪，韦精卫，石德顺
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（广西大学 动物繁殖研究所,广西 南宁530005）
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摘要：
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水牛卵母细胞体外成熟22h后，分别用盲吸法、点击法和纺锤体成像系统（Spindle-View System）去核。结果，三者的去核率分别为65.1%、81.8%和95.0%，差异极显著（P<001）。以水牛胎儿成纤维细胞为供体，通过核移植构建的重构胚的融合率、分裂率和囊胚发育率，在上述3种方法之间均没有显著差异（P>0.05）。认为点击法是一种较为简单、实用和有效的去核方法。 |
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关键词：
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水牛；卵母细胞；去核；核移植；点击法 |
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中图分类号：S 814.8 文献标识码：
A 文章编号：1673-4696(2006)06-0493-04 |
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Comparison of enucleation protocols of buffalo oocytes |
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CHEN Zi-hong, YANG Su-fang, XIE Ying, LI Ri-cong, WEI Jing-wei, SHI De-shun |
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（Animal Reproduction Institute, Guangxi University,Nanning 530005, China） |
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Abstract: Buffalo oocytes matured in vitro for 22 h were enucleated by blind-sucking, point-hitting and Spindle-View System, respectively. The enucleation rate of oocytes by the Spindle-View System(95%) was significantly(P<0.01) higher than that achieved by blind-sucking(65.1%) and the point-hitting(81.8%). However, there was no significant difference in fusion ration, cleavage rate and blastocyst yield of the reconstructed embryos among the three enucleation methods(P>0.05). Results indicated that the point-hitting can be employed for enucleation of oocytes in consideration of the costs of the Spindle-View System. |
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Key words: buffalo; oocyte; enucleation; nuclear transplantation; point-hitting |
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