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铜对生长猪肝中IGF-Ⅰ基因mRNA表达的影响
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郑鑫1, 2，刘国文1,3，杨连玉1,2 ，李家奎1, 4，夏清娟2 ，郝德威2，王哲1
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(1.吉林大学 畜牧兽医学院，吉林 长春 130026； 2.吉林农业大学 动物科技学院，吉林 长春 130118； 3.内
蒙古民族大学 动物科技学院，内蒙古 通辽 028042； 4.华中农业大学 动物科技学院，湖北 武汉 430070)
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摘要：
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采用生长试验、免疫放射分析和定量PCR方法，观察了生长猪血液中类胰岛素生长因子(IGF-Ⅰ)及其结合蛋白(IGFBP3)数量的变化和IGF-Ⅰ基因在肝中表达量的变化。结果显示，每1kg饲料中添加125～250mg铜，能够上调IGF-Ⅰ基因的表达量，显著提高猪的平均日增重，促进生长猪循环血液中IGF-Ⅰ浓度的增加。试验结果证实，铜是通过促进生长激素-胰岛素样生长因子轴相关因子的合成和分泌来发挥促生长作用的。 |
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关键词：
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铜；猪；肝；类胰岛素生长因子；基因表达 |
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中图分类号：S 816.72 文献标识码：
A 文章编号：1673-4696(2006)06-0497-05 |
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Effect of copper on insulin-like growth factor and gene
expression of IGF-Ⅰ mRNA in liver of growing pigs |
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ZHENG Xin1,2 ,LIU
Guo-wen1,3, YANG Lian-yu1,2 , LI
Jia-kui1,4,
XIA Qing-juan2,HAO De-wei2,WANG
Zhe1 |
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(1.College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062,China；
2．College of Animal Science and Technology，Jilin Agricultural University，Changchun 130118，China；
3．College of Animal Science and Technology, Nei Monggol University for Nationalities, Tongliao 028042, China;
4．College of Animal Science and Technology,Huazhong Agricultural University, Wuhan 430070, China) |
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Abstract: The experiment was conducted in order to explore the effect of copper on growth correlation factor from the level of integer and gene expression, such as porcine liver IGF-Ⅰ, in order to elucidate the modulation action of copper on liver and somatotropin axis correlation factor and internal mechanism of growth-promoting of copper. Quantitative changes of gene expression of IGF-Ⅰ and IGFBP in blood were detected by growth promoting experiment, RIA and quantitative PCR. These results indicated that the addition of copper(125-250mg/kg) in food improved the IGF-Ⅰ gene expression, significantly increased the average body weight gain.These results indicated that copper stimulates growth by the synthesis and secretion of some factors of axis. |
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Key words: copper；porcine；liver; insulin-like growth factor；gene expression |
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