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一株新的鸽源基因Ⅵ型新城疫病毒的分离鉴定
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邵朝纲1，单松华2，陈佳一1
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（1.湖州师范学院，浙江 湖州313000； 2.上海出入境检验检疫局，上海200135）
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摘要：
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从进口鸽中分离到1株鸽Ⅰ型副黏病毒，经测定，其鸡胚平均死亡时间(MDT)为59.2h,鸡脑内接种致病指数（ICPI）为1.92，属于速发型新城疫病毒。通过RT-PCR，扩增出了该分离毒株F基因的重要功能区片段，经测序分析，其裂解位点的氨基酸序列为112RRQKR117F,符合新城疫强毒裂解位点特征。用blastn和blastx分别搜索GenBank的核酸和蛋白数据库，发现它与2株鸽Ⅰ型副黏病毒具有99%的同源性，系统发育进化树分析为基因Ⅵ型。表明，该病毒为1株新的强毒力鸽源基因Ⅵ型新城疫病毒。 |
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关键词：
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鸽Ⅰ型副黏病毒； 新城疫病毒； 基因Ⅵ型 |
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中图分类号：S 852.659.5 文献标识码：
A 文章编号：1673-4696(2006)07-0543-04 |
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Isolation and identification of a new pigeon genotype Ⅵ strain of
Newcastle disease virus |
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SHAO Chao-gang1, SHAN Song-hua2, CHEN
Jia-yi1 |
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(1.Huzhou Teachers College, Huzhou 313000, China; 2.Shanghai Entry-Exit Inspection and
Quarantine Bureau, Shanghai 200135,China ) |
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Abstract: A strain of pigeon paramyxovirus typeⅠ isolated from an imported pigeon represented the highly pathogenic NDV because its mean death time(MDT) of chicken embryos was 59.2 hours and its intracerebral pathogenicity index(ICPI) in one-day-old chicken was 1.92.The important fragment of F gene was amplified by RT-PCR,followed by sequencing and analysis.The motif of its cleave site was112RRQKR117F which was consistent with that of velogenic strains of NDV. blastn with the Nucleotide database and blastx with the Protein database in GenBank showed that it had 99% of identity with twostrains of pigeon paramyxovirus type Ⅰ,and phylogenetic analysis indicated that it belonged to the genotypeⅥ.Results showed that the isolated virus strain represented a new virulent pigeon genotype Ⅵ NDV. |
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Key words: pigeon paramyxovirus typeⅠ； NDV； genotype Ⅵ |
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