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热应激对植入前牛胚胎发育的影响
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李守军1,范秀军2,田文儒3
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（1.华南农业大学 兽医学院，广东 广州510642； 2.中国科学院 动物研究所 生殖生物学国家重点实验室，
北京100080； 3.莱阳农学院 动物科学系，山东 莱阳265200）
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摘要：
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采集屠宰母牛卵巢和输卵管中的卵母细胞，进行体外受精，受精卵继续在体外培养。将发育3d的牛胚胎于42℃下分别培养0.5、2.0和4.0h，与对照组（39℃）牛胚胎相比，接触最高热应激（42℃，4h）处理的胚胎，约50%的胚胎发育速度明显下降。而处理组牛胚胎发育到第9d的囊胚数、细胞数及内细胞团与滋养外胚层比率与对照组差异不显著(P>0.05)。表明，温度升高对牛囊胚期胚胎的发育没有明显损害。对9d的囊胚进行基因性别分析发现，正常体外培养的雄胚发育快于雌胚，而在发育第3d受42℃热应激后，到囊胚期生存的胚胎性别比率发生改变，雌胚比率高于雄胚，表明雌性胚胎比雄性胚胎具有更强的抗热应激能力。 |
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关键词：
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热应激；发育；植入前胚胎 |
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中图分类号：S 852.1文献标识码：
A 文章编号：1673-4696(2006)07-0566-07 |
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Effect of heat shock on bovine pre-implantation embryos |
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LI Shou-jun1, FAN Xiu-jun2, TIAN
Wen-ru3 |
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（1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
2.State Key Laboratory of Reproductive Biology/Institute of Zoology, Chinese Academy of Sciences, Beijing 100080,
China; 3.Faculty of Animal Science and Veterinary Medicine, Laiyang Agricultural College, Laiyang 265200, China) |
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Abstract: Effect of elevated temperature on the early bovine embryo in vitro were analyzed in order to determine its impact on the development rate, cell lineage allocation, survival rate based on genetic sex. Exposure of bovine embryos on day 3 of development to 42℃ for 0.5h, 2.0h, and 4.0h lowered their potential to reach blastocyst stage on day 9 of development compared to control（39.0℃). However, cell counts and inner cell mass to trophectoderm ratios of the embryos exposed to elevated temperature were not different from the control, suggesting that the developmental potential of the embryos which survive to blastocyst stage were not overtly impaired. Analysis of the genetic sex of the control embryos which deve-loped to the hatched blastocyst stage in spite of exposure to 42.0℃ on day 3 of development showed a shift in sex ratio in favour of females, indicating that XX embryos may be better able to withstand heat stress. |
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Key words: heat shock; development; pre-implantation |
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