|
|
|
以乳酸杆菌为载体的口蹄疫病毒VP1基因
DNA疫苗猪体免疫试验
|
|
|
|
李云岗1，2，田夫林2，王会波3，马慧玲2,陈静2,李新生1,高凤山1，夏春1
|
|
|
|
（1.中国农业大学 动物医学院，北京100094； 2.山东省畜牧兽医总站，山东 济南250022；
3.济南市动物防检疫总站，山东 济南250002）
|
|
|
|
摘要：
|
|
给猪口服和肌肉注射以乳酸杆菌为载体的口蹄疫病毒VP1基因DNA疫苗(L.acido SFMD-1)，以正向间接血凝试验（IHA）和MTT方法分别检测了免疫后FMDV VP1抗体的动态变化和特异性T细胞增殖反应情况，并与商用FMD油佐剂疫苗、裸质粒FMDV VP1基因DNA疫苗诱导的特异性免疫抗体水平进行了比较。结果显示，口服组猪在免疫后第21d抗体效价达到了1∶27.7，而肌肉注射组猪为1∶23.3。加强免疫后2周，口服免疫组抗体水平下降到1∶25.3，到第3周快速上升到1∶28；与此相对应，肌肉途径免疫组猪抗体效价缓慢地从1∶25.3上升到1∶26.7。口服途径和肌肉注射途径的刺激指数（SI）分别为1.93和2.00，2种免疫途径都可以诱导特异性T细胞增殖反应。证实，该疫苗能够在猪体诱发VP1特异性T细胞和B细胞反应，以乳酸杆菌为载体是DNA免疫和预防猪口蹄疫的一种极有前景的方法。 |
|
关键词：
|
|
乳酸杆菌; 口蹄疫; DNA疫苗 |
|
中图分类号：S 852.659.6 文献标识码：
A 文章编号：1673-4696(2006)08-0601-05 |
|
|
Vaccination of pigs against foot-and-mouth disease virus with a DNA
vaccine containing VP1 gene using Lactobacillus acidophilus as carrier |
|
|
|
LI Yun-gang1,2,TIAN Fu-lin2,WANG
Hui-bo3,MA Hui-ling2,
CHEN Jing2,LI Xin-sheng1,GAO Feng-shan1,XIA
Chun1 |
|
|
|
(1.College of Veterinary Medicine,China Agricultural University,Beijing 100094,China;
2.Shandong Province Husbandry and Veterinary Service Station,Jinan 250022,China;
3.Jinan Animal Diseases Prevention and Quarantine General Service Station,Jinan 250002,China)
|
|
|
Abstract: Pigs were inoculated with a DNA vaccine encoding FMDV VP1 gene using Lactobacillus aci-dophilus as carrier (L.acido SFMD-1).Anti-FMDV VP1-specific antibody and T cell proliferation response were measured by the indirect haemagglutination test and the MTT assay,respectively. Compared with the intramuscular injection routes,the oral administration group showed a significant higher antibody level on day 21,and the titer reached 1∶27.7,whereas the titer in the intramuscular injection route was 1∶23.3.On week 2 post-booster,the titer of the oral administration group decreased to 1∶25.3,and then rose rapidly to 1∶28 on next week; whereas the counterpart route from 1∶25.3 to 26.7.The stimulation index for immunization via oral and the intramuscular routes reached 1.93 and 2.00,respectively.The results demonstrated clearly that vaccine could elicit VP1-specific T cell and B cell response in swine,and L.acidophilus as a carrier was a potent tool for DNA immunization and defense against FMDV. |
|
|
|
Key words: Lactobacillus acidophilus; foot-and-mouth disease; DNA vaccine |
|
|
|
|
|
《中国兽医科学》 ©
版权所有
Chinese Veterinary Science
zgsykx@zgsykx.com |
