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辽宁省禽大肠埃希氏菌血清型的调查
及其致病性试验
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李玉文，李惠兰，齐景文，闫明媚，于长泳，李胜杰，张雷
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(辽宁省动物防疫站，辽宁 沈阳110161）
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摘要：
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通过病原菌分离、血清型鉴定及本动物致病性试验对辽宁省禽大肠埃希氏菌的主要血清型分布与致病性进行了研究。2001～2005年从该省14个市的病死禽和死胚中分离出大肠埃希氏菌226株，血清型鉴定105株，其中O78型占29.52%（31/105），O109型占10.48%（11/105）。结果，O78、O109血清型是流行于该省致病性禽大肠埃希氏菌的优势血清型。随机选取49株分离菌,以每株1×107CFU的量于气管内给6只1日龄SPF鸡接种,根据接种后7d内死亡和病变的情况,确定高致病株、中度致病株和低致病株，它们分别占755%、184%和61%。结果表明，所有分离菌株均有致病性,并在国内首次分离出禽O109型大肠埃希氏菌。 |
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关键词：
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禽；大肠埃希氏菌；血清型；致病性 |
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中图分类号：S 852.612 文献标识码：
A 文章编号：1673-4696(2006)08-0630-04 |
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Investigation of serotype distribution and pathogenicity of
avian Escherichia coli in Liaoning Province |
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LI Yu-wen,LI Hui-lan,QI Jing-wen,YAN Ming-mei,YU Chang-yong,LI Sheng-jie,ZHANG Lei |
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（Animal Epidemic Prevention Station of Liaoning Province,Shenyang 110161,China） |
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Abstract: Strains of Escherichia coli in Liaoning Province were isolated and identified and their pathogenicity was examined.From 2001 to 2005，226 strains of E.coli were obtained by isolation from the di-seased or dead chickens and dead embryo from 14 prefectures of the Province,and 105 strains out of them were serotyped,with 29.52%(31/105) being O78 and 10.48%(11/105) being O109 .Results indicated that O78 and O109 were the predominant serotypes in Liaoning Province.Pathogenicity of 49 E.coli strains selected randomly from the 105 strains were examined endotracheally in 1 day-old SPF chickens.Six birds per isolate were inoculated intracheally with 0.1mL of a 24h Luria-bertani(LB) broth culture containing 1×107CFU E.coli.Based on mortality and pathological changes during a 7-day post-inoculation period,755%（37/49）,18.4%（9/49）,6.1%（3/49） were characterized as high,intermediate and low pathogeni-city,respectively.These results showed that the all E.coli isolates were pathogenic,in which 75.5% of strains were high pathogenic.Avian E.coli serotype O109 and its pathogenicity were reported the first time in China. |
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Key words: avian; Escherichia coli; serotype; pathogenicity |
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