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牛源环孢子虫的形态学特征观察
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肖淑敏1，2，李国清1，李韦华1，周荣琼1
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（1.华南农业大学 兽医学院，广东 广州 510642；2.河南理工大学 安全科学与工程学院，河南 焦作 454000）
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摘要：
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对首次在乳牛粪便中发现的疑似环孢子虫卵囊的形状、大小、抗酸染色和孢子化等形态学特征进行了观察，探讨了该原虫的纯化方法。结果显示，牛源环孢子虫卵囊呈球形，直径6～10μm。直接涂片时可见卵囊内含折光性较强的成团小球体，呈桑椹胚状，淡绿色。碘液染色不着色。经改良抗酸染色后，卵囊着色变化较大，呈淡红色、深红色或不着色。新排出的未孢子化卵囊培养后可观察到孢子化的卵囊，内含2个孢子囊，每个孢子囊内含有2个折光性较强的子孢子。这些特征与人或其他动物的环孢子虫一致。环孢子虫感染阳性的粪便经Sheather’s蔗糖漂浮法浓集后，再经蔗糖密度梯度离心，可以得到较为纯净的卵囊。 |
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关键词：
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环孢子虫样卵囊；乳牛；形态学 |
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中图分类号：S 853.723文献标识码：
A 文章编号：1673-4696(2006)08-0639-04 |
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Morphologic characterization of Cyclospora-like
oocyst from dairy cattle |
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XIAO Shu-min1，2,LI Guo-qing1,LI
Wei-hua1,ZHOU Rong-qiong1 |
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(1.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China；
2.School of Safety Science and Engineering,Henan Polytechnic University,Jiaozuo 454000,China) |
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Abstract: Cyclospora-like oocysts were found in dairy cattle feces.Using wet mount slides,the oocyst walls appeared as well-defined non-refractile spheres measuring 6 to 10μm in diameter,within each unsporulated oocyst was a central morula-like structure containing a variable number of inclusions.At a magnification of ×1000,the inclusions were refractile,exhibiting a greenish tinge.Oocysts were stained variably using a modified acid-fast stains but colorless in saline wet mounts.Oocysts were concentrated and purified by sucrose flotation.Purified oocysts were suspended in equal volumes of 5% potassium dichromate solution to sporulate.The sporulated oocyst holds 2 sporocysts and each sporocyst contains 2 sporozoites,i.e.each oocyst contains a total of 4 sporozoites.All the above phenotypic characteristics were simi-lar to those described previously in human being.This is the first cyclosporan to be described in dairy cattle. |
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Key words: Cyclospora-like oocyst; dairy cattle; morphology |
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