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毒害艾美球虫二次感染雏鸡外周血液免疫功能的变化
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郑世民，刘晶，胡京友，高雪丽
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（东北农业大学 动物医学学院，黑龙江 哈尔滨150030）
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摘要：
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将180只14日龄雏鸡随机分为毒害艾美球虫(Eimeria necatrix)初次感染组、二次感染组和对照组，应用免疫SPA菌体花环、间接ELISA及细胞培养技术和四甲基偶氮唑盐（MTT）测定法对相关免疫学指标进行了检测，以研究毒害艾美球虫二次感染对雏鸡外周血液免疫功能变化的影响。结果显示，毒害艾美球虫二次感染雏鸡外周血液T、B淋巴细胞数量及其对ConA或PMA的增殖反应和血清IgG、IgM、IgA免疫球蛋白含量均不同程度地高于未感染毒害艾美球虫的对照组雏鸡。证实毒害艾美球虫二次感染雏鸡外周血液的细胞免疫和体液免疫功能均明显提高。 |
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关键词：
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毒害艾美球虫；二次感染；雏鸡；外周血液； 淋巴细胞数量和功能；免疫球蛋白 |
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中图分类号：S 852.743.4：Q 786文献标识码：
A 文章编号：1673-4696(2006)08-0643-04 |
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Changes of immune function of peripheral blood of chickens
secondarily-infected with Eimeria necatrix |
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ZHENG Shi-min,LIU Jing,HU Jing-you,GAO Xue-li |
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(College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030，China) |
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Abstract: One hundred and eighty 14-days-old chickens were divided randomly into coccidia primary infection group (I1),coccidia secondary infection group (I2),and control group(C).Immune indexes were examined by using immune staphytococcal protein A (SPA) rosette test,indirect enzyme-linked immunosorbent assay（IELISA），cellular culture technique and MTT method.The number and proliferative responses of T and B lymphocytes to ConA or phorbol myristate acetate (PMA),contents of IgG,IgM and IgA in peripheral blood of 14-days-old chickens secondarily infected with E.necatrix were elevated diffe-rent extent compared with that of the control.The cellular and fluid immune function of peripheral blood of chickens secondarily-infected with E.necatrix were significantly increased. |
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Key words: Eimeria necatrix；secondary infection；chicken；peripheral blood；number and function of T and B cells；immunoglobulin |
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