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新生仔猪大肠埃希氏菌性腹泻K88ac-ST1-LTB三价
基因工程灭活疫苗生产工艺的研究
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卫广森1，2 ，陆承平3 ，陈溥言3
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（1．中国农业科学院 兰州兽医研究所，甘肃 兰州730046； 2.中农威特生物科技股份有限公司,
甘肃 兰州730046； 3.南京农业大学 动物医学院,江苏 南京210095）
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摘要：
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以实验室工艺为基础，用发酵罐培养，对培养基、诱导剂及诱导条件、通气量、菌液灭活条件等进行了筛选优化。结果表明，重组菌株BL21(DE3)(pXK88acST3LT5)培养的最适培养基为改良LB培养基；乳糖为适合于工业化生产的诱导剂，其最适浓度为100mmol/L，诱导时间不低于6h；2×105mL发酵罐培养的最佳通气量为500L/min；培养菌液的灭活条件为按菌液总量加入4mL/L甲醛溶液，37℃灭活48h。按上述工业化生产条件生产疫苗5批，检验结果均安全有效。 |
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关键词：
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新生仔猪；大肠埃希氏菌；三价基因工程疫苗；乳糖诱导剂；生产工艺 |
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中图分类号：S 855.12 文献标识码：
A 文章编号：1673-4696(2006)09-0707-06 |
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Studies on commercial producing process of K88ac-ST1-LTB trivalent
gene engineering inactivated vaccine against colibacillus
diarrhea of newborn piglets |
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WEI Guang-sen1,2,LU Cheng-ping3,CHEN
Pu-yan3 |
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(1.Lanzhou Veterinary Research Institute，Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;
2.China Veterinary Biological Science and Technology Co.,Ltd,Lanzhou 730046,China;
3.College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095,China) |
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Abstract: On the basis of laboratory process, the culture medium, inducement, ventilation volume and inactivated condition were optimized under industry producing conditions. In consideration of the stability of BL21(DE3) (pXK88acST3LT5) bacterial strain and the cost of production, the improved LB medium was selected as the optimal one. The lactose of 100mmol/L was the best inducement. In the 200 liter fermenter, the optimal ventilation volume was 500L/min. The optimal concentration of formaldehyde was 4mL/L and the inactivating condition was 48 hours at 37℃. Five batches of vaccine produced using the optimal commercial conditions were safe and effective, indicating that the commercial producing process was scientifically sound and commercially practical. |
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Key words: newborn piglet; Escherichia coli; K88ac-ST1-LTB；lactose inducement；producing process |
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