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兔脂肪间质干细胞的分离培养与鉴定
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马勇江1,2，杨学义2，窦忠英2，李玉谷1，张媛1
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(1.华南农业大学 兽医学院，广东 广州510642； 2.西北农林科技大学 陕西省干细胞工程技术
研究中心，陕西 杨凌712100）
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摘要：
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取兔腹股沟皮下脂肪组织，用Ⅰ型胶原酶消化，分离培养脂肪间质干细胞(MSCs)，并用免疫组化和体外诱导分化方法对其表面分子标志和多向分化潜能进行鉴定。结果显示，兔脂肪组织中能够分离培养出脂肪MSCs，该类细胞表达CD29、CD44和CD105，不表达CD34、CD45 及HLA-DR表面分子标志，并具有可分化为脂肪细胞、神经细胞和成骨细胞的多向分化潜能，证实兔脂肪组织中存在具有多向分化潜能的MSCs。 |
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关键词：
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脂肪组织；间质干细胞；兔 |
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中图分类号：Q 813.11文献标识码：
A 文章编号：1673-4696(2006)09-0733-05 |
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Cultivation and identification of adipose tissue-derived
mesenchymal stem cells from rabbits |
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MA Yong-jiang1，2, YANG Xue-yi2, DOU
Zhong-ying2, LI Yu-gu1, ZHANG Yuan1 |
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(1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
2.Shaanxi Center of Stem Cell Engineering and Technology, Northwest Sci-Tech University of
Agriculture and Forestry, Yangling 712100, China) |
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Abstract: Mesenchymal stem cells isolated from rabbit inguinal fat pads were digested with collagenase type I, and their molecular characteristics and multi-lineage potential were identified by immunohistochemical methods and induction in vitro. In results, rabbit adipose tissue derived mesenchymal stem cells could express CD29, CD44 and CD105, whereas they did not express CD34, CD45 or HLA-DR. They had the ability to differentiate into adipocytes, neurocytes and osteoblasts. The results provided significant evidence that there are multipotential mesenchymal stem cells in rabbit adipose tissue. |
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Key words: adipose tissue; mesenchymal stem cells; rabbit |
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