|
|
|
鸡IL-2和IL-15基因真核表达质粒对禽流感
疫苗的免疫增强作用
|
|
|
|
胡慧琼1,王红宁2，1,袁斌1,周生1,李晓英1,黄勇1,柳萍1,徐永莉1
|
|
|
|
（1.四川农业大学 动物科技学院 动物预防医学生物工程实验室，四川 雅安625014；
2.四川大学 生命科学学院，四川 成都610064）
|
|
|
|
摘要：
|
|
根据GenBank中的鸡IL-2和IL-15序列设计了2对引物，采用RT-PCR法从山地乌骨鸡外周血淋巴细胞RNA中克隆了IL-2和IL-15基因，与pcDNA3.1连接构建了真核表达质粒pDNAIL-2和pDNAIL-15，研究了其对禽流感灭活疫苗的免疫增强作用。结果显示，免疫后第10d，pDNAIL-15+AI灭活疫苗组和pDNAIL-2+AI灭活疫苗组HI抗体水平显著高于AI灭活疫苗组（P<0.05）。免疫后第10d，pDNAIL-15+AI灭活疫苗组CD4+淋巴细胞百分含量显著高于pDNAIL-2+AI灭活疫苗和AI灭活疫苗组（P<0.05）；免疫后第23d，pDNAIL-2+AI灭活疫苗组CD4+淋巴细胞百分率显著高于pDNAIL-15+AI灭活疫苗和AI灭活疫苗组（P<0.05）。证实pDNAIL-2和pDNAIL-15能提高禽流感灭活疫苗的免疫效果。 |
|
关键词：
|
|
鸡白细胞介素-2；鸡白细胞介素-15；禽流感疫苗；HI抗体 |
|
中图分类号：S 852.659.5
文献标识码：
A 文章编号：1673-4696(2006)10-0805-06 |
|
|
Immunoenhancement of eukaryotic expression plasmids with chicken
IL-2 or IL-15 genes on H5 subtype avian influenza vaccine |
|
|
|
HU Hui-qiong1, WANG Hong-ning2,1, YUAN
Bin1,ZHOU Sheng1,
LI Xiao-ying1, HUANG Yong1, LIU Ping1, XU
Yong-li1 |
|
|
|
(1.Laboratory of Animal Preventive Medicine and Bio-engineering, College of Animal Science and Technology, Sichuan
Agricultural University, Ya’an 625014，China; 2.College of Life Science,Sichuan University,Chengdu 610064,China) |
|
|
Abstract: Two pairs of specific primers for chicken interleukin-2(CHIL-2) and interleukin-15(CHIL-15) genes were designed according to the published gene sequences of CHIL-2 and CHIL-15. The eukar-yotic expression plasmids with CHIL-2 gene or CHIL-15 gene, named pDNAIL-2 and pDNAIL-15, were constructed by subcloning CHIL-2 gene or CHIL-15 gene into pcDNA3.1（+）, respectively. The immunoenhancement of pDNAIL-2 and pDNAIL-15 on H5 subtype avian influenza vaccine was observed by detection of HI antibody titers and percentages of CD4+ and CD8+. In results, on day 10 post-inoculation(PI), the HI antibody titers in the pDNAIL-15+AI vaccine group and the pDNAIL-2+AI vaccine group were significantly higher than those in the AI vaccine group（P<0.05）. The percentage of CD4+ in the pDNAIL-15+AI vaccine group was significantly higher than that in the pDNAIL-2+AI vaccine group and the AI vaccine group on day 10 PI（P<0.05）. The percentage of CD4+ in the pDNAIL-2+AI vaccine group was significantly higher than that in the pDNAIL-15+AI vaccine group and the AI vaccine on day 23 PI（P<0.05）.These results indicated that pDNAIL-2 and pDNAIL-15 have adjuvant effects on the AI vaccine. |
|
|
|
Key words: chicken interleukin 2 ;chicken interleukin 15; avian influenza vaccine; HI antibody |
|
|
|
|
|
《中国兽医科学》 ©
版权所有
Chinese Veterinary Science
zgsykx@zgsykx.com |
