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猪链球菌2型三种重组表达蛋白对BALB/c
小鼠的免疫保护性评价
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王华1,2，王君玮1，陈义平1，张维1，徐天刚1，赵云玲1，王志亮1
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(1.中国动物卫生与流行病学中心ABSL-3实验室，山东 青岛266114；
2.扬州大学 兽医学院，江苏 扬州225009）
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摘要：
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将150只BALB/c小鼠随机分成5组。用猪链球菌2型pET32a-Sly、pET32a-38ku和pET32a-Sly-38ku表达的重组蛋白制备的疫苗,以及猪链球菌2型全菌灭活疫苗分别免疫A、B、C、D组小鼠，E组为生理盐水对照。首免后第2周加强免疫1次，二免后第2周，用致死量强毒猪链球菌2型四川分离株和江苏分离株以及马链球菌兽疫亚种安徽分离株攻毒，观察攻毒后对小鼠的免疫保护率。结果，A组与C、D组对猪链球菌2型感染的保护率差异显著（P＜0.05），而C组与D组之间保护率无显著差异；A、B、C、D组之间对马链球菌兽疫亚种安徽分离株感染的保护率差异不显著；各免疫组的保护率极显著高于对照组。结果表明，3种重组疫苗具有良好的免疫原性，能对强毒株猪链球菌2型的攻击感染起到保护作用。 |
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关键词：
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猪链球菌2型；重组蛋白；BALB/c小鼠；免疫原性 |
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中图分类号：S 852.611
文献标识码：
A 文章编号：1673-4696(2007)10-0863-04 |
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Assessment of protective efficacy of three recombinant protein
vaccines against Streptococcus suis type 2 in BALB/c mice |
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WANG Hua1,2,WANG Jun-wei1,CHEN
Yi-ping1,ZHANG Wei1,XU Tian-gang1，
ZHAO Yun-ling1，WANG Zhi-liang1 |
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(1.ABSL-3 Laboratory,China Animal Health and Epidemiology Center,Qingdao 266114,China;
2.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China) |
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Abstract: The 150 BALB/c mice were divided randomly into 5 groups.Mice in Group A,B and C were vaccinated with the vaccines developed from the proteins expressed from plasmids pET32a-Sly,pET32a-38ku and pET32a-Sly-38ku,respectively,mice in Group D or E were injected with the dead Streptococcus suis type 2 vaccine or normal saline solution.On week 2 post-vaccination,all the mice were inoculated by booster immunization(BI),respectively.On week 2 post-BI,all the mice were challenged with a lethal dose of S.suis type 2 Sichuan strain and Jiangsu strain and Streptococcus equi subsp.zooepidemicus Anhui strain,respectively,and the protection rates of the vaccines were evaluated post-challenge.The protection rate(PR) against S.suis type 2 and S.equi subsp.zooepidemicus in Group A was significantly different from those in Group C and D,but no significant difference between Group C and D.The PRs against S.equi subsp.zooepidemicus were not significantly different among Group A,B,C and D.The PRs against all the strains in Group A,B,C and D were significantly higher than that in Group E.These results showed that the three recombinant protein vaccines had good immunogenicity and were able to induce protective immune responses in mice. |
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Key words: Streptococcus suis 2;recombinant protein;BALB/c mouse;immunogenicity |
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